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Anti-DAPK2 TRUEMAB Antibody clone 1B10
TrueMAB Antibodies - Made against Authentic Protein Antigens
Also for DAPK2 (NM_014326)
|Full length human recombinant protein of human DAPK2 (NP_055141) produced in HEK293T cell.|
|WB, IF, IP, FC
||WB 1:2000, IF 1:100, FLOW 1:100, IP 2ug/500ul
|PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
|Homo sapiens death-associated protein kinase 2 (DAPK2)|
Entrez Gene 23604 Human
|This gene encodes a protein that belongs to the serine/threonine protein kinase family. This protein contains a N-terminal protein kinase domain followed by a conserved calmodulin-binding domain with significant similarity to that of death-associated protein kinase 1 (DAPK1), a positive regulator of programmed cell death. Overexpression of this gene was shown to induce cell apoptosis. It uses multiple polyadenylation sites. [provided by RefSeq]. |
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DAPK2 (RC216274, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DAPK2.
Anti-DAPK2 mouse monoclonal antibody (TA501204) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY DAPK2(RC216274).
Immunoprecipitation(IP) of DAPK2 by using TrueMab monoclonal anti-DAPK2 antibodies (Negative control: IP without adding anti-DAPK2 antibody.). For each experiment, 500ul of DDK tagged DAPK2 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-DAPK2 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
HEK293T cells transfected with either RC216274 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-DAPK2 antibody(TA501204), and then analyzed by flow cytometry.
Flow cytometric Analysis of Hela cells, using anti-DAPK2 antibody(TA501204),(Red), compared to a nonspecific negative control antibody,(Blue).
Flow cytometric Analysis of Jurkat cells, using anti-DAPK2 antibody(TA501204),(Red), compared to a nonspecific negative control antibody,(Blue).