Anti-PDE4A Antibody, clone 7B11

Also for PDE4A (NM_006202)

Gene Name	Homo sapiens phosphodiesterase 4A, cAMP-specific (PDE4A), transcript variant 4
Synonyms:	DPDE2; PDE4; PDE46
Immunogen	Full length human recombinant protein of human PDE4A (NP_006193) produced in HEK293T cell.
Buffer	PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Clone Name	clone 7B11	Isotype	IgG2b
Species Reactivity	Human	Concentration	0.5~1.0 mg/ml (Lot Dependent)
Purification	Purified from mouse ascites fluids by affinity chromatography (Protein A or G Sepharose)
Guaranteed Application *	WB, IHC, IF, FC	Suggested Dilutions	WB 1:2000, IHC 1:50, IF 1:100, FLOW 1:100
Background	Cyclic nucleotides are important second messengers that regulate and mediate a number of cellular responses to extracellular signals, such as hormones, light, and neurotransmitters. Cyclic nucleotide phosphodiesterases (PDEs) regulate the cellular concentrations of cyclic nucleotides and thereby play a role in signal transduction. PDE4A is a class IV cAMP-specific PDE
Related Pathway	Signaling by GPCR

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WB Image

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDE4A (RC207765, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDE4A .

IHC Image

Immunohistochemical staining of paraffin-embedded Human colon tissue using anti-PDE4A mouse monoclonal antibody. (TA501201, Dilution 1:50)

IHC Image

Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-PDE4A mouse monoclonal antibody. (TA501201, Dilution 1:50)

IHC Image

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PDE4A mouse monoclonal antibody. (TA501201, Dilution 1:50)

IHC Image

Immunohistochemical staining of paraffin-embedded Human endometrium tissue using anti-PDE4A mouse monoclonal antibody. (TA501201, Dilution 1:50)

IHC Image

Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-PDE4A mouse monoclonal antibody. (TA501201, Dilution 1:50)

IHC Image

Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-PDE4A mouse monoclonal antibody. (TA501201, Dilution 1:50)

IF Image

Anti-PDE4A mouse monoclonal antibody (TA501201) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PDE4A (RC207765 ).

IF Image

Immunofluorescent staining of HT29 cells using anti-PDE4A mouse monoclonal antibody (TA501201).

FC Image

HEK293T cells transfected with either RC207765 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PDE4A antibody(TA501201), and then analyzed by flow cytometry.

FC Image

Flow cytometric Analysis of Jurkat cells, using anti-PDE4A antibody(TA501201),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).

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Anti-PDE4A TrueMAB Antibody clone 7B11

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