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Anti-PDE4A TRUEMAB Antibody clone 3D4
TrueMAB Antibodies - Made against Authentic Protein Antigens
Also for PDE4A (NM_006202)
|Full length human recombinant protein of human PDE4A (NP_006193) produced in HEK293T cell.|
|WB, IHC, IF, FC
||WB 1:2000, IHC 1:50, IF 1:100, FLOW 1:100
|PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
|Homo sapiens phosphodiesterase 4A, cAMP-specific (PDE4A), transcript variant 4|
|DPDE2; PDE4; PDE46|
|Cyclic nucleotides are important second messengers that regulate and mediate a number of cellular responses to extracellular signals, such as hormones, light, and neurotransmitters. Cyclic nucleotide phosphodiesterases (PDEs) regulate the cellular concentrations of cyclic nucleotides and thereby play a role in signal transduction. PDE4A is a class IV cAMP-specific PDE |
Signaling by GPCR
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDE4A (RC207765, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDE4A .
Immunohistochemical staining of paraffin-embedded Human liver tissue using anti-PDE4A mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA501198, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-PDE4A mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA501198, Dilution 1:50)
Anti-PDE4A mouse monoclonal antibody (TA501198) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PDE4A (RC207765 ).
Immunofluorescent staining of HT29 cells using anti-PDE4A mouse monoclonal antibody (TA501198).
HEK293T cells transfected with either RC207765 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PDE4A antibody(TA501198), and then analyzed by flow cytometry.
Flow cytometric Analysis of Jurkat cells, using anti-PDE4A antibody(TA501198),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).