The nucleoside diphosphate (NDP) kinases (EC 184.108.40.206) are ubiquitous enzymes that catalyze transfer of
gamma-phosphates, via a phosphohistidine intermediate, between nucleoside and dioxynucleoside tri- and diphosphates.The enzymes are products of the nm23 gene family, which includes NME4
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NME4 (RC202603, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NME4.