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Home Antibody All anti-NIT2 antibodies
KO-Validated

Anti-NIT2 TRUEMAB Antibody Clone OTI3D5

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations Customer Reviews Product Documents
SKU Description Amount Price Availability*  
TA501097 anti-NIT2 mouse monoclonal antibody, clone OTI3D5 (formerly 3D5) 100ul $325 In Stock
LC402761 NIT2 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF501097 Carrier-free (BSA/glycerol-free) NIT2 mouse monoclonal antibody, clone OTI3D5 (formerly 3D5) 100ug $450 4 Weeks
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WB(3)
IHC(3)
IF(1)
FC(1)

OriGene Data

ImmunogenFull length human recombinant protein of human NIT2 (NP_064587) produced in HEK293T cell.
Clone NameClone OTI3D5 IsotypeIgG2b
Species ReactivityHuman Concentration0.79 mg/ml
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsWB 1:2000, IHC 1:50, IF 1:100, Flow 1:100
Predicted MW Explanation 30.4kDa
BufferPBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens nitrilase family member 2 (NIT2)
Alternative NameHEL-S-8a; HEL-S-8a
Database LinkNP_064587
Entrez Gene 56954 Human
FunctionHas a omega-amidase activity. The role of omega-amidase is to remove potentially toxic intermediates by converting alpha-ketoglutaramate and alpha-ketosuccinamate to biologically useful alpha-ketoglutarate and oxaloacetate, respectively. Overexpression decreases the colony-forming capacity of cultured cells by arresting cells in the G2 phase of the cell cycle
Related Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.


WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NIT2 (RC210660, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NIT2.
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WB Image
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-NIT2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).(1:200)
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WB Image
Equivalent amounts of cell lysates (10 ug per lane) of wild-type 293T cells (WT, Cat# LC810293T) and NIT2-Knockout 293T cells (KO, Cat# LC812235) were separated by SDS-PAGE and immunoblotted with anti-NIT2 monoclonal antibody TA501097,(1:500). Then the blotted membrane was stripped and reprobed with anti-HSP90AB1 antibody (TA500494) as a loading control.
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IHC Image
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-NIT2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501097, Dilution 1:50)
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IHC Image
Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-NIT2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501097, Dilution 1:50)
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IHC Image
Immunohistochemical staining of paraffin-embedded liver tissue within the normal limits using anti-NIT2 mouse monoclonal antibody. (TA501098, Dilution 1:50; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min)
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IF Image
Anti-NIT2 mouse monoclonal antibody (TA501097) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY NIT2(RC210660).
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FC Image
HEK293T cells transfected with either pCMV6-ENTRY NIT2(RC210660)(Red) or empty vector control plasmid(Blue) were immunostained with anti-NIT2 mouse monoclonal(TA501097), and then analyzed by flow cytometry.
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