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Home Antibody All anti-ARHGDIA antibodies
KO-Validated

Anti-ARHGDIA TRUEMAB Antibody Clone OTI1F2

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations Customer Reviews Product Documents
SKU Description Amount Price Availability*  
TA501089 ARHGDIA (RhoGDI) mouse monoclonal antibody, clone OTI1F2 (formerly 1F2) 100ul $379 In Stock
LC401371 ARHGDIA HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF501089 Carrier-free (BSA/glycerol-free) ARHGDIA mouse monoclonal antibody, clone OTI1F2 (formerly 1F2) 100ug $450 4 Weeks
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WB(3)
FC(3)
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Also for ARHGDIA (NM_004309)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human ARHGDIA (NP_004300) produced in HEK293T cell.
Clone NameClone OTI1F2 IsotypeIgG1
Species ReactivityHuman, Dog, Rat, Monkey Concentration1.00mg/ml
Guaranteed Application *WB, FC Suggested DilutionsWB 1:200~1000, FLOW 1:100
Predicted MW Explanation 23.0 kDa
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from cell culture supernatant by affinity chromatography

Reference Data

Target NameRho GDP dissociation inhibitor alpha
Alternative NameGDIA1|HEL-S-47e|NPHS8|RHOGDI|RHOGDI-1
Database LinkNP_004300
Entrez Gene 396 Human
Entrez Gene 360678 Rat
Entrez Gene 714752 Monkey
Entrez Gene 475924 Dog
FunctionAplysia Ras-related homologs (ARHs), also called Rho genes, belong to the RAS gene superfamily encoding small guanine nucleotide exchange (GTP/GDP) factors. The ARH proteins may be kept in the inactive, GDP-bound state by interaction with GDP dissociation inhibitors, such as ARHGDIA (Leffers et al., 1993 [PubMed 8262133]).[supplied by OMIM]
Related PathwayDruggable Genome Neurotrophin signaling pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.


WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ARHGDIA (RC200902, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ARHGDIA.
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WB Image
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ARHGDIA monoclonal antibody.
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WB Image
Equivalent amounts of cell lysates (10 ug per lane) of wild-type 293T cells (WT, Cat# LC810293T) and ARHGDIA-Knockout 293T cells (KO, Cat# LC811309) were separated by SDS-PAGE and immunoblotted with anti-ARHGDIA monoclonal antibody TA501089,(1:500). Then the blotted membrane was stripped and reprobed with anti-HSP90AB1 antibody (TA500494) as a loading control.
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FC Image
HEK293T cells transfected with either RC200902 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-ARHGDIA antibody(TA501089), and then analyzed by flow cytometry.
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FC Image
Flow cytometric Analysis of Hela cells, using anti-ARHGDIA antibody(TA501089),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
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FC Image
Flow cytometric Analysis of Jurkat cells, using anti-ARHGDIA antibody(TA501089),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
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