Anti-PIM2 Antibody, clone 8B4

Also for PIM2 (NM_006875)

OriGene Data

Immunogen	Full length human recombinant protein of human PIM2 (NP_006866) produced in HEK293T cell.
Clone Name	clone 8B4	Isotype	IgG2a
Species Reactivity	Human	Concentration	0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *	WB, IHC, IF, IP, FC	Suggested Dilutions	WB 1:500, IHC 1:50, IF 1:100, FLOW 1:100, IP 2ug/500ul
Buffer	PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification	Purified from mouse ascites fluids by affinity chromatography (Protein A or G Sepharose)

Reference Data

Target Name	Homo sapiens pim-2 oncogene (PIM2)
Alternative Name
Database Link	NP_006866
Function	This gene encodes a protooncogene that acts as a serine/threonine protein kinase. Studies determined the encoded protein functions to prevent apoptosis and to promote cell survival.
Related Pathway

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WB Image

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PIM2 (RC201933, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PIM2.

IHC Image

Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-PIM2 mouse monoclonal antibody. (TA501062)

IHC Image

Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-PIM2 mouse monoclonal antibody. (TA501062)

IF Image

Anti-PIM2 mouse monoclonal antibody (TA501062) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PIM2(RC201933).

IP Image

Immunoprecipitation(IP) of PIM2 by using TrueMab monoclonal anti-PIM2 antibodies (Negative control: IP without adding anti-PIM2 antibody.). For each experiment, 500ul of DDK tagged PIM2 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-PIM2 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.

FC Image

HEK293T cells transfected with either RC201933 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PIM2 antibody(TA501062), and then analyzed by flow cytometry.

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OriGene offers bulk sizes of TrueMAB antibodies in both the BSA/glycerol formulation and the carrier-free (BSA/glycerol-free) formulation.

Anti-PIM2 TrueMAB Antibody clone 8B4

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

OriGene Data

Reference Data