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Anti-TUBA8 TrueMAB Antibody clone 2E9
TrueMAB Antibodies - Made against Authentic Protein Antigens
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Also for TUBA8 (NM_018943)
|Full length human recombinant protein of human TUBA8(NP_061816) produced in HEK293T cell.|
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IF, FC
||WB 1:2000; IF 1:100;
|PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography (Protein A or G Sepharose)
|Homo sapiens tubulin, alpha 8 (TUBA8), transcript variant 1|
|Microtubules are cylindrical tubes of 20-25 nm in diameter. They are composed of protofilaments which are in turn composed of alpha- and beta-tubulin polymers. Each microtubule is polarized, at one end alpha-subunits are exposed (-) and at the other beta-subunits are exposed (+). Microtubules act as a scaffold to determine cell shape, and provide a backbone for cell organelles and vesicles to move on, a process that requires motor proteins. The major microtubule motor proteins are kinesin, which generally moves towards the (+) end of the microtubule, and dynein, which generally moves towards the (-) end. Microtubules also form the spindle fibers for separating chromosomes during mitosis.|
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TUBA8 (RC211175, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TUBA8.
Anti-TUBA8 mouse monoclonal antibody (TA501038) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY TUBA8(RC211175).
HEK293T cells transfected with either pCMV6-ENTRY TUBA8(RC211175)(Red) or empty vector control plasmid(Blue) were immunostained with anti-TUBA8 mouse monoclonal(TA501038), and then analyzed by flow cytometry.