Its interaction with CUL3 suggests that it may act as a substrate adapter in some E3 ligase complex. Does not affect the function of Kv channel Kv2.1/KCNB1, Kv1.2/KCNA2, Kv4.2/KCND2 and Kv3.4/KCNC4
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KCTD5 (RC200180, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KCTD5.
HEK293T cells transfected with either pCMV6-ENTRY KCTD5(RC200180)(Red) or empty vector control plasmid(Blue) were immunostained with anti-KCTD5 mouse monoclonal(TA501035), and then analyzed by flow cytometry.
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