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Anti-LIPG TRUEMAB Antibody Clone OTI1B8

TrueMAB™ Antibodies - Made against Authentic Protein Antigens


Specifications Citations Customer Reviews Product Documents
SKU Description Amount Price Availability*  
TA501002 LIPG mouse monoclonal antibody, clone OTI1B8 (formerly 1B8) 100ul 325
In Stock
LC401821 LIPG HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF501002 Carrier-free (BSA/glycerol-free) LIPG mouse monoclonal antibody, clone OTI1B8 (formerly 1B8) 100ug $450 3 Days
Conjugation is available for this antibody: choose conjugation type
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OriGene Data

ImmunogenFull length human recombinant protein of human LIPG (NP_006024) produced in HEK293T cell.
Clone NameClone OTI1B8 IsotypeIgG1
Species ReactivityHuman Concentration0.85 mg/ml
Guaranteed Application *WB, IHC, IF, IP, FC Suggested DilutionsWB 1:2000, IHC 1:50, IF 1:100, FLOW 1:100, IP 2ug/500ul
Predicted MW Explanation 54.6 kDa
BufferPBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens lipase, endothelial (LIPG)
Alternative NameEDL; EL; PRO719
Database LinkNP_006024
Entrez Gene 9388 Human
FunctionThe protein encoded by this gene has substantial phospholipase activity and may be involved in lipoprotein metabolism and vascular biology. This protein is designated a member of the TG lipase family by its sequence and characteristic lid region which provides substrate specificity for enzymes of the TG lipase family. [provided by RefSeq].
Related PathwaySecreted ProteinDruggable Genome Glycerolipid metabolismMetabolic pathways

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LIPG (RC209248, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LIPG.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-LIPG monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).(1:200)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-LIPG mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501002)
Anti-LIPG mouse monoclonal antibody (TA501002) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY LIPG(RC209248).
Immunoprecipitation(IP) of LIPG by using TrueMab monoclonal anti-LIPG antibodies (Negative control: IP without adding anti-LIPG antibody.). For each experiment, 500ul of DDK tagged LIPG overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-LIPG antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
HEK293T cells transfected with either RC209248 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-LIPG antibody(TA501002), and then analyzed by flow cytometry.


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