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Home Antibody All anti-SIL1 antibodies

Anti-SIL1 TRUEMAB Antibody Clone OTI3E3

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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SKU Description Amount Price Availability*  
TA500997
  • anti-SIL1 mouse monoclonal antibody, clone OTI3E3 (formerly 3E3)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC402924) , 20ug Explanation
100ul $325 In Stock
CF500997
  • Carrier-free (BSA/glycerol-free) SIL1 mouse monoclonal antibody, clone OTI3E3 (formerly 3E3)
100ug $450 3-4 weeks

Buy any antibody of 100ul or more, get a free package of 3 loading control antibody samples. View Details Add to Shopping Cart

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WB(2)
IHC(4)
IF(2)
FC(1)

OriGene Data

ImmunogenFull length human recombinant protein of human SIL1 (NP_071909) produced in HEK293T cell.
Clone NameClone OTI3E3 IsotypeIgG1
Species ReactivityHuman Concentration0.56 mg/ml
Guaranteed Application *WB, IHC, IF, FC Suggested Dilutions IHC 1:100~500, WB: 1:200
Predicted MW Explanation 52.1kDa
BufferPBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens SIL1 nucleotide exchange factor (SIL1), transcript variant 2
Alternative NameBAP; MSS; ULG5
Database LinkNP_071909
Entrez Gene 64374 Human
FunctionThis gene encodes a resident endoplasmic reticulum (ER), N-linked glycoprotein with an N-terminal ER targeting sequence, 2 putative N-glycosylation sites, and a C-terminal ER retention signal. This protein functions as a nucleotide exchange factor for another unfolded protein response protein. Mutations in this gene have been associated with Marinesco-Sjogren syndrome. Alternate transcriptional splice variants have been characterized.
Related PathwaySecreted ProteinTransmembraneProtease

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SIL1 (RC211850, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SIL1.
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Western blot analysis of extracts (10ug) from 3 different cell lines by using anti-SIL1 monoclonal antibody.(1:200)
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Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-SIL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500997, Dilution 1:50)
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Immunohistochemical staining of paraffin-embedded liver tissue within the normal limits using anti-SIL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500997, Dilution 1:50)
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Immunohistochemical staining of paraffin-embedded Carcinoma of liver tissue using anti-SIL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500997, Dilution 1:50)
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-SIL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500997, Dilution 1:50)
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Anti-SIL1 mouse monoclonal antibody (TA500997) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SIL1(RC211850).
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Immunofluorescent staining of HT29 cells using anti-SIL1 mouse monoclonal antibody (TA500997).
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HEK293T cells transfected with either pCMV6-ENTRY SIL1(RC211850)(Red) or empty vector control plasmid(Blue) were immunostained with anti-SIL1 mouse monoclonal(TA500997), and then analyzed by flow cytometry.
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