Anti-APP Antibody, clone 5F1

Also for APP (NM_000484)

OriGene Data

Immunogen	Full length human recombinant protein of human APP (NP_000475) produced in HEK293T cell.
Clone Name	clone 5F1	Isotype	IgG2a
Species Reactivity	Human	Concentration	0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *	WB, IHC, IF	Suggested Dilutions	WB 1:2000, IHC 1:50, IF 1:100,
Buffer	PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification	Purified from mouse ascites fluids by affinity chromatography (Protein A or G Sepharose)

Reference Data

Target Name	Homo sapiens amyloid beta (A4) precursor protein (APP), transcript variant 1
Alternative Name	AAA; ABETA; ABPP; AD1; APPI; CTFgamma; CVAP; PN-II; PN2
Database Link	NP_000475
Function	This gene encodes a cell surface receptor and transmembrane precursor protein that is cleaved by secretases to form a number of peptides. Some of these peptides are secreted and can bind to the acetyltransferase complex APBB1/TIP60 to promote transcriptional activation, while others form the protein basis of the amyloid plaques found in the brains of patients with Alzheimer disease. Mutations in this gene have been implicated in autosomal dominant Alzheimer disease and cerebroarterial amyloidosis (cerebral amyloid angiopathy). Multiple transcript variants encoding several different isoforms have been found for this gene.
Related Pathway	Delta-Notch Signaling Pathway Hemostasis

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WB Image

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY APP (RC221339, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-APP.

IHC Image

Immunohistochemical staining of paraffin-embedded Human colon tissue using anti-APP mouse monoclonal antibody. (TA500991)

IHC Image

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-APP mouse monoclonal antibody. (TA500991)

IHC Image

Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-APP mouse monoclonal antibody. (TA500991)

IHC Image

Immunohistochemical staining of paraffin-embedded Human liver tissue using anti-APP mouse monoclonal antibody. (TA500991)

IHC Image

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-APP mouse monoclonal antibody. (TA500991)

IHC Image

Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-APP mouse monoclonal antibody. (TA500991)

IHC Image

Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-APP mouse monoclonal antibody. (TA500991)

IF Image

Anti-APP mouse monoclonal antibody (TA500991) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY APP(RC221339).

IF Image

Immunofluorescent staining of HT29 cells using anti-APP mouse monoclonal antibody (TA500991).

Conjugation Services
OriGene provides conjugation services of TrueMAB antibodies with the following molecules:

HRP
Biotin
PE
FITC
DyLight® 488
DyLight® 550
DyLight® 594
DyLight® 633

* DyLight is a trademark of Thermo-Fisher Scientific

Bulk Order
OriGene offers bulk sizes of TrueMAB antibodies in both the BSA/glycerol formulation and the carrier-free (BSA/glycerol-free) formulation.

Anti-APP TrueMAB Antibody clone 5F1

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

OriGene Data

Reference Data