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Anti-PSMC3 TRUEMAB Antibody Clone OTI9F3
TrueMAB Antibodies - Made against Authentic Protein Antigens
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Also for PSMC3 (NM_002804)
|Full length human recombinant protein of human PSMC3(NP_002795) produced in HEK293T cell.|
|WB, IHC, IF, FC
||WB 1:2000, IHC 1:50, IF 1:100, Flow 1:100
|PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
|Homo sapiens proteasome 26S subunit, ATPase 3 (PSMC3)|
Entrez Gene 5702 Human
Entrez Gene 475980 Dog
|The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits.Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the ATPase subunits, a member of the triple-A family of ATPases that have chaperone-like activity. This subunit may compete with PSMC2 for binding to the HIV tat protein to regulate the interaction between the viral protein and the transcription complex. A pseudogene has been identified on chromosome 9.|
|Transcription FactorsDruggable Genome Proteasome|
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PSMC3 (RC201790, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PSMC3.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PSMC3 monoclonal antibody.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-PSMC3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500922, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-PSMC3mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500922, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded prostate tissue within the normal limits using anti-PSMC3mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500922, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded Carcinoma of bladder tissue using anti-PSMC3mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500922, Dilution 1:50)
Anti-PSMC3 mouse monoclonal antibody (TA500922) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PSMC3(RC201790).
HEK293T cells transfected with either pCMV6-ENTRY PSMC3(RC201790)(Red) or empty vector control plasmid(Blue) were immunostained with anti-PSMC3 mouse monoclonal(TA500922), and then analyzed by flow cytometry.
Flow cytometric analysis of Jurkat cells, using anti-PSMC3 antibody(TA500922),(Red) compared to a nonspecific negative control antibody(TA50011)(Blue).