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Anti-RALBP1 TRUEMAB Antibody Clone OTI6G10
TrueMAB Antibodies - Made against Authentic Protein Antigens
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Also for RALBP1 (NM_006788)
|Full length human recombinant protein of human RALBP1 (NP_006779) produced in HEK293T cell.|
|WB, IHC, IF, IP, FC
||WB 1:2000, IHC 1:50, IF 1:100, Flow 1:100, IP: 4ug/mL
|PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
|Homo sapiens ralA binding protein 1 (RALBP1)|
|RIP1; RLIP1; RLIP76|
Entrez Gene 10928 Human
|Can activate specifically hydrolysis of GTP bound to RAC1 and CDC42, but not RALA. Mediates ATP-dependent transport of S-(2,4-dinitrophenyl)-glutathione (DNP-SG) and doxorubicin (DOX) and is the major ATP-dependent transporter of glutathione conjugates of electrophiles (GS-E) and DOX in erythrocytes. Can catalyze transport of glutathione conjugates and xenobiotics, and may contribute to the multidrug resistance phenomenon. Serves as a scaffold protein that brings together proteins forming an endocytotic complex during interphase and also with CDC2 to switch off endocytosis, One of its substrates would be EPN1/Epsin|
| Pathways in cancerPancreatic cancer|
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RALBP1 (RC201524, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RALBP1.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-RALBP1 monoclonal antibody.
Western blot analysis of extracts (10ug) from 10 Human tissue by using anti-RALBP1 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).
Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-RALBP1mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500915, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded liver tissue within the normal limits using anti-RALBP1mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500915, Dilution 1:50)
Anti-RALBP1 mouse monoclonal antibody (TA500915) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RALBP1(RC201524).
Immunoprecipitation(IP) of RALBP1 by using TrueMab monoclonal anti-RALBP1 antibodies (Negative control: IP without adding anti-RALBP1 antibody.). For each experiment, 500ul of DDK tagged RALBP1 overexpression lysates (at 1:5 dilution with HEK293T lysate),
HEK293T cells transfected with either pCMV6-ENTRY RALBP1(RC201524)(Red) or empty vector control plasmid(Blue) were immunostained with anti-RALBP1 mouse monoclonal(TA500915), and then analyzed by flow cytometry.