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Anti-LOX TRUEMAB Antibody Clone 5C4
TrueMAB Antibodies - Made against Authentic Protein Antigens
|Human recombinant protein fragment corresponding to amino acids 22-168 of human LOX (NP_002308) produced in HEK293T cell.|
|Human , Dog , Rat
|WB, IHC, IF, FC
||WB 1:2000, IHC 1:50, IF 1:100, FLOW 1:100,
|PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
|Homo sapiens lysyl oxidase (LOX), transcript variant 1|
|The protein encoded by this gene is an extracellular copper enzyme that initiates the crosslinking of collagens and elastin. The enzyme catalyzes oxidative deamination of the epsilon-amino group in certain lysine and hydroxylysine residues of collagens and lysine residues of elastin. In addition to crosslinking extracellular matrix proteins, the encoded protein may have a role in tumor suppression. Defects in this gene are a cause of autosomal recessive cutis laxa type I (CL type I). Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]. |
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LOX (RC213323, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LOX.
Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-LOX monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Immunohistochemical staining of paraffin-embedded Human colon tissue using anti-LOX mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA500859)
Anti-LOX mouse monoclonal antibody (TA500859) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY LOX(RC213323).
HEK293T cells transfected with either RC213323 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-LOX antibody(TA500859), and then analyzed by flow cytometry.
Flow cytometric Analysis of Hela cells, using anti-LOX antibody(TA500859),(Red), compared to a nonspecific negative control antibody,(Blue).
Flow cytometric Analysis of Jurkat cells, using anti-LOX antibody(TA500859),(Red), compared to a nonspecific negative control antibody,(Blue).