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Home Antibody All anti-BRAF antibodies

Anti-BRAF TRUEMAB Antibody clone 1D4

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA500852
  • anti-BRAF mouse monoclonal antibody, clone 1D4
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC401382) , 20ug Explanation
100ul 325 In Stock
CF500852
  • Carrier-free (BSA/glycerol-free) BRAF mouse monoclonal antibody, clone 1D4
100ug $450 3 Days
This antibody qualifies for Season of Giving promotion: Earn $50 Amazon gift card (use promo code SPQ12081) or donation to Cancer.org (promo code SPQ12082) with $600 purchase of UltraMAB or TrueMAB. Click for details
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WB(1)
IHC(3)
IF(1)
FC(1)
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Also for BRAF (NM_004333)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human BRAF (NP_004324) produced in HEK293T cell.
Clone Nameclone 1D4 IsotypeIgG1
Species ReactivityHuman Concentration0.64 mg/ml
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsWB 1:2000, IHC 1:50, IF 1:100, Flow 1:100
Predicted MW Explanation 84.4 kDa
BufferPBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens B-Raf proto-oncogene, serine/threonine kinase (BRAF)
Alternative NameB-RAF1; BRAF1; NS7; RAFB1
Database LinkNP_004324
Entrez Gene 673 Human
FunctionThis gene encodes a protein belonging to the raf/mil family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. Mutations in this gene have also been associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung. A pseudogene, which is located on chromosome X, has been identified for this gene.
Related PathwayProtein KinaseDruggable Genome MAPK signaling pathwayErbB signaling pathwayChemokine signaling pathwaymTOR signaling pathwayVascular smooth muscle contractionFocal adhesionMore Pathways >>

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BRAF (RC211013, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BRAF.
IHC Image
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-BRAF mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500852, Dilution 1:50)
IHC Image
Immunohistochemical staining of paraffin-embedded liver tissue using anti-BRAFmouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500852, Dilution 1:50)
IHC Image
Immunohistochemical staining of paraffin-embedded Carcinoma of liver tissue using anti-BRAFmouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500852, Dilution 1:50)
IF Image
Anti-BRAF mouse monoclonal antibody (TA500852) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BRAF(RC211013).
FC Image
HEK293T cells transfected with either pCMV6-ENTRY BRAF(RC211013)(Red) or empty vector control plasmid(Blue) were immunostained with anti-BRAF mouse monoclonal(TA500852), and then analyzed by flow cytometry.

 

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