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Anti-ATP5B TRUEMAB Antibody Clone OTI7F4
TrueMAB Antibodies - Made against Authentic Protein Antigens
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Also for ATP5B (NM_001686)
|Full length human recombinant protein of human ATP5B (NP_001677) produced in HEK293T cell.|
|Human, Dog, Monkey
|WB, IHC, IF, FC
||WB 1:1000, IHC 1:50, IF 1:100, Flow 1:100
|PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
|Homo sapiens ATP synthase, H+ transporting, mitochondrial F1 complex, beta polypeptide (ATP5B)|
|ATPMB; ATPSB; HEL-S-271|
Entrez Gene 506 Human
Entrez Gene 713284 Monkey
Entrez Gene 403669 Dog
|This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis,utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the beta subunit of the catalytic core.|
|Druggable Genome Oxidative phosphorylationMetabolic pathwaysAlzheimer's diseaseParkinson's diseaseHuntington's disease|
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ATP5B (RC201638, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ATP5B.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ATP5B monoclonal antibody.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-ATP5B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500849, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded Carcinoma of liver tissue using anti-ATP5Bmouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500849, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of endometrium tissue using anti-ATP5Bmouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500849, Dilution 1:50)
Anti-ATP5B mouse monoclonal antibody (TA500849) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ATP5B(RC201638).
Immunofluorescent staining of HepG2 cells using anti-ATP5B mouse monoclonal antibody (TA500849).
HEK293T cells transfected with either pCMV6-ENTRY ATP5B(RC201638)(Red) or empty vector control plasmid(Blue) were immunostained with anti-ATP5B mouse monoclonal(TA500849), and then analyzed by flow cytometry.
Flow cytometric analysis of Jurkat cells, using anti-ATP5B antibody(TA500849),(Red) compared to a nonspecific negative control antibody(TA50011)(Blue).