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Home Antibody All anti-ATP5B antibodies

Anti-ATP5B TRUEMAB Antibody Clone OTI1B10

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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SKU Description Amount Price Availability*  
TA500837 anti-ATP5B mouse monoclonal antibody, clone OTI1B10 (formerly 1B10) 100ul $379 In Stock
LC400637 ATP5B HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF500837 Carrier-free (BSA/glycerol-free) ATP5B mouse monoclonal antibody, clone OTI1B10 (formerly 1B10) 100ug $450 3-4 weeks
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WB(2)
IHC(3)
IF(1)
FC(2)
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Also for ATP5B (NM_001686)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human ATP5B (NP_001677) produced in HEK293T cell.
Clone NameClone OTI1B10 IsotypeIgG1
Species ReactivityHuman, Dog, Rat, Monkey Concentration0.71 mg/ml
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsWB 1:2000, IHC 1:50, IF 1:100, Flow 1:100
Predicted MW Explanation 56.6 kDa
BufferPBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens ATP synthase, H+ transporting, mitochondrial F1 complex, beta polypeptide (ATP5B)
Alternative NameATPMB; ATPSB; HEL-S-271
Database LinkNP_001677
Entrez Gene 506 Human
Entrez Gene 171374 Rat
Entrez Gene 713284 Monkey
Entrez Gene 403669 Dog
FunctionThis gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis,utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the beta subunit of the catalytic core.
Related PathwayDruggable Genome Oxidative phosphorylationMetabolic pathwaysAlzheimer's diseaseParkinson's diseaseHuntington's disease

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ATP5B (RC201638, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ATP5B.
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ATP5B monoclonal antibody.
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-ATP5B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500837, Dilution 1:50)
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Immunohistochemical staining of paraffin-embedded liver tissue within the normal limits using anti-ATP5Bmouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500837, Dilution 1:50)
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Immunohistochemical staining of paraffin-embedded Carcinoma of liver tissue using anti-ATP5Bmouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500837, Dilution 1:50)
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Anti-ATP5B mouse monoclonal antibody (TA500837) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ATP5B(RC201638).
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Flow cytometric analysis of Hela cells, using anti-ATP5B antibody(TA500837),(Red) compared to a nonspecific negative control antibody(TA50011)(Blue).
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Flow cytometric analysis of Jurkat cells, using anti-ATP5B antibody(TA500837),(Red) compared to a nonspecific negative control antibody(TA50011)(Blue).
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