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Home Antibody All anti-AKT2 antibodies

Anti-AKT2 TRUEMAB Antibody Clone OTI4G1

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA500831 AKT2 mouse monoclonal antibody, clone OTI4G1 (formerly 4G1) 100ul $325 In Stock
LC419836 AKT2 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF500831 Carrier-free (BSA/glycerol-free) AKT2 mouse monoclonal antibody, clone OTI4G1 (formerly 4G1) 100ug $450 3-4 weeks
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WB(1)
IP(1)

OriGene Data

ImmunogenFull length human recombinant protein of human AKT2 (NP_001617) produced in HEK293T cell.
Clone NameClone OTI4G1 IsotypeIgG1
Species ReactivityHuman Concentration0.53 mg/ml
Guaranteed Application *WB, IP Suggested DilutionsWB 1:2000, IP 2ug/500ul
Predicted MW Explanation 55.6 kDa
BufferPBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens v-akt murine thymoma viral oncogene homolog 2 (AKT2), transcript variant 1
Alternative NameHIHGHH; PKBB; PKBBETA; PRKBB; RAC-BETA
Database LinkNP_001617
Entrez Gene 208 Human
FunctionThis gene is a putative oncogene encoding a protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains. The gene was shown to be amplified and overexpressed in 2 of 8 ovarian carcinoma cell lines and 2 of 15 primary ovarian tumors. Overexpression contributes to the malignant phenotype of a subset of human ductal pancreatic cancers. The encoded protein is a general protein kinase capable of phophorylating several known proteins. [provided by RefSeq].
Related PathwayProtein KinaseES Cell Differentiation/IPSDruggable Genome MAPK signaling pathwayErbB signaling pathwayChemokine signaling pathwaymTOR signaling pathwayApoptosisVEGF signaling pathwayMore Pathways >>

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AKT2 (RC217733, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AKT2.
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Immunoprecipitation(IP) of AKT2 by using TrueMab monoclonal anti-AKT2 antibodies (Negative control: IP without adding anti-AKT2 antibody.). For each experiment, 500ul of DDK tagged AKT2 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-AKT2 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
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