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Home Antibody All anti-GBE1 antibodies

Anti-GBE1 TRUEMAB Antibody Clone 1D11

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations (1) Related Products Product Documents
SKU Description Amount Price Availability*  
TA500829
  • GBE1 mouse monoclonal antibody, clone 1D11
  • FREE positive control: HEK293T cell transient overexpression lysate (LC400056) , 20ug
100ul 379
$284
In Stock
CF500829
  • Carrier-free (BSA/glycerol-free) GBE1 mouse monoclonal antibody, clone 1D11
100ug $450 3-4 weeks
Add to Shopping Cart
WB(3)
IP(1)
IHC(3)
IF(2)
FC(2)
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Also for GBE1 (NM_000158)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human GBE1 (NP_000149) produced in HEK293T cell.
Clone NameClone 1D11 IsotypeIgG1
Species ReactivityHuman Concentration1.1mg/ml
Guaranteed Application *WB, IHC, IF, IP, FC Suggested DilutionsWB 1:500~2000, IHC 1:50, IF 1:50~100, FLOW 1:100, IP 2ug/500ul
BufferPBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens glucan (1,4-alpha-), branching enzyme 1 (GBE1)
Alternative NameAPBD; GBE; GSD4
Database LinkNP_000149
FunctionThe protein encoded by this gene is a glycogen branching enzyme that catalyzes the transfer of alpha-1,4-linked glucosyl units from the outer end of a glycogen chain to an alpha-1,6 position on the same or a neighboring glycogen chain. Branching of the chains is essential to increase the solubility of the glycogen molecule and, consequently, in reducing the osmotic pressure within cells. Highest level of this enzyme are found in liver and muscle. Mutations in this gene are associated with glycogen storage disease IV (also known as Andersen's disease). [provided by RefSeq].
Related Pathway

* Shipping is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GBE1 (RC204152, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GBE1.
WB Image
Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-GBE1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
WB Image
Western blot analysis of extracts (10ug) from 10 Human tissue by using anti-GBE1 monoclonal antibody at 1:500 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).
IHC Image
Immunohistochemical staining of paraffin-embedded Human colon tissue using anti-GBE1 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA500829)
IHC Image
Immunohistochemical staining of paraffin-embedded Human Ovary tissue using anti-GBE1 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA500829)
IHC Image
Immunohistochemical staining of paraffin-embedded Human endometrium tissue using anti-GBE1 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA500829)
IF Image
Anti-GBE1 mouse monoclonal antibody (TA500829) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY GBE1(RC204152).
IF Image
Immunofluorescent staining of HepG2 cells using anti-GBE1 mouse monoclonal antibody (TA500829).
IP Image
Immunoprecipitation(IP) of GBE1 by using TrueMab monoclonal anti-GBE1 antibodies (Negative control: IP without adding anti-GBE1 antibody.). For each experiment, 500ul of DDK tagged GBE1 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-GBE1 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
FC Image
HEK293T cells transfected with either RC204152 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-GBE1 antibody(TA500829), and then analyzed by flow cytometry.
FC Image
Flow cytometric Analysis of Jurkat cells, using anti-GBE1 antibody(TA500829),(Red), compared to a nonspecific negative control antibody,(Blue).

 

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