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Anti-ACO2 TRUEMAB Antibody Clone OTI3G8
TrueMAB Antibodies - Made against Authentic Protein Antigens
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Also for ACO2 (NM_001098)
|Full length human recombinant protein of human ACO2 (NP_001089) produced in HEK293T cell.|
|WB, IHC, IF
||WB 1:2000, IHC 1:50, IF 1:100,
|PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
|Homo sapiens aconitase 2, mitochondrial (ACO2)|
Entrez Gene 50 Human
|The protein encoded by this gene belongs to the aconitase/IPM isomerase family. It is an enzyme that catalyzes the interconversion of citrate to isocitrate via cis-aconitate in the second step of the TCA cycle. This protein is encoded in the nucleus and functions in the mitochondrion. It was found to be one of the mitochondrial matrix proteins that are preferentially degraded by the serine protease 15(PRSS15), also known as Lon protease, after oxidative modification.|
| Citrate cycle (TCA cycle)Glyoxylate and dicarboxylate metabolismMetabolic pathways|
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ACO2 (RC204307, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ACO2.
Western blot analysis of extracts (10ug) from 10 Human tissue by using anti-ACO2 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-ACO2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500824, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-ACO2mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500824, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded Ovary tissue within the normal limits using anti-ACO2mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500824, Dilution 1:50)
Anti-ACO2 mouse monoclonal antibody (TA500824) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ACO2(RC204307).
Immunofluorescent staining of HepG2 cells using anti-ACO2 mouse monoclonal antibody (TA500824).