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Anti-ID3 TRUEMAB Antibody Clone OTI7B6
TrueMAB Antibodies - Made against Authentic Protein Antigens
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|Full length human recombinant protein of human ID3 (NP_002158) produced in HEK293T cell.|
|WB, IHC, IF
||WB 1:2000, IHC 1:50, IF 1:100,
|PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
|Homo sapiens inhibitor of DNA binding 3, dominant negative helix-loop-helix protein (ID3)|
Entrez Gene 3399 Human
|This gene encodes a protein belonging to the raf/mil family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. Mutations in this gene have also been associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung. A pseudogene, which is located on chromosome X, has been identified for this gene. |
|ES Cell Differentiation/IPSTranscription FactorsDruggable Genome TGF-beta signaling pathway|
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ID3 (RC200583, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ID3.
Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-ID3mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500811, Dilution 1:50)
Anti-ID3 mouse monoclonal antibody (TA500811) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ID3(RC200583).
Immunofluorescent staining of HeLa cells using anti-ID3 mouse monoclonal antibody (TA500811).
Immunofluorescent staining of HT29 cells using anti-ID3 mouse monoclonal antibody (TA500811).