Anti-HNRNPF Antibody, clone 5F5

Also for HNRNPF (NM_004966)

Gene Name	Homo sapiens heterogeneous nuclear ribonucleoprotein F (HNRNPF), transcript variant 3
Synonyms:	HNRPF; mcs94-1; OK/SW-cl.23
Immunogen	Full length human recombinant protein of human HNRNPF (NP_004957) produced in HEK293T cell.
Buffer	PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Clone Name	clone 5F5	Isotype	IgG2a
Species Reactivity	Human	Concentration	0.5~1.0 mg/ml (Lot Dependent)
Purification	Purified from mouse ascites fluids by affinity chromatography (Protein A or G Sepharose)
Guaranteed Application *	WB, IHC, IF, FC	Suggested Dilutions	WB 1:2000, IHC 1:50, IF 1:50~100, FLOW 1:100
Background	This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins that complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and regulate alternative splicing, polyadenylation, and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has three repeats of quasi-RRM domains that bind to RNAs which have guanosine-rich sequences. This protein is very similar to the family member hnRPH. Multiple alternatively spliced variants, encoding the same protein, have been identified.
Related Pathway

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WB Image

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HNRNPF (RC205100, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HNRNPF.

IHC Image

Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-HNRNPF mouse monoclonal antibody. (TA500804)

IHC Image

Immunohistochemical staining of paraffin-embedded Human liver tissue using anti-HNRNPF mouse monoclonal antibody. (TA500804)

IHC Image

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-HNRNPF mouse monoclonal antibody. (TA500804)

IHC Image

Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-HNRNPF mouse monoclonal antibody. (TA500804)

IHC Image

Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-HNRNPF mouse monoclonal antibody. (TA500804)

IF Image

Anti-HNRNPF mouse monoclonal antibody (TA500804) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY HNRNPF(RC205100).

IF Image

Immunofluorescent staining of HepG2 cells using anti-HNRNPF mouse monoclonal antibody (TA500804).

FC Image

Flow cytometric Analysis of Hela cells, using anti-HNRNPF antibody(TA500804),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).

FC Image

Flow cytometric Analysis of Jurkat cells, using anti-HNRNPF antibody(TA500804),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).

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Anti-HNRNPF TrueMAB Antibody clone 5F5

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