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Home Antibody All anti-HNRNPF antibodies

Anti-HNRNPF TRUEMAB Antibody clone 5F5

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA500804
  • HNRNPF (hnRNP F) mouse monoclonal antibody, clone 5F5
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC401544) , 20ug Explanation
100ul
30ul
379 In Stock
CF500804
  • Carrier-free (BSA/glycerol-free) HNRNPF mouse monoclonal antibody, clone 5F5
100ug $450 3-4 weeks
This antibody qualifies for Season of Giving promotion: Earn $50 Amazon gift card (use promo code SPQ12081) or donation to Cancer.org (promo code SPQ12082) with $600 purchase of UltraMAB or TrueMAB. Click for details
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WB(2)
IHC(5)
IF(2)
FC(2)
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Also for HNRNPF (NM_004966)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human HNRNPF (NP_004957) produced in HEK293T cell.
Clone Nameclone 5F5 IsotypeIgG2a
Species ReactivityHuman Concentration0.61 mg/ml
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsWB 1:2000, IHC 1:50, IF 1:50~100, FLOW 1:100
Predicted MW Explanation 45.7 kDa
BufferPBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens heterogeneous nuclear ribonucleoprotein F (HNRNPF), transcript variant 3
Alternative NameHNRPF; mcs94-1; OK/SW-cl.23
Database LinkNP_004957
Entrez Gene 3185 Human
FunctionThis gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins that complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and regulate alternative splicing, polyadenylation, and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has three repeats of quasi-RRM domains that bind to RNAs which have guanosine-rich sequences. This protein is very similar to the family member hnRPH. Multiple alternatively spliced variants, encoding the same protein, have been identified.
Related Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HNRNPF (RC205100, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HNRNPF.
WB Image
Western blot analysis of extracts (10ug) from 10 Human tissue by using anti-HNRNPF monoclonal antibody at 1:1000 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).
IHC Image
Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-HNRNPF mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500804)
IHC Image
Immunohistochemical staining of paraffin-embedded Human liver tissue using anti-HNRNPF mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500804)
IHC Image
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-HNRNPF mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500804)
IHC Image
Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-HNRNPF mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500804)
IHC Image
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-HNRNPF mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500804)
IF Image
Anti-HNRNPF mouse monoclonal antibody (TA500804) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY HNRNPF(RC205100).
IF Image
Immunofluorescent staining of HepG2 cells using anti-HNRNPF mouse monoclonal antibody (TA500804).
FC Image
Flow cytometric Analysis of Hela cells, using anti-HNRNPF antibody(TA500804),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
FC Image
Flow cytometric Analysis of Jurkat cells, using anti-HNRNPF antibody(TA500804),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).

 

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