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Home Antibody All anti-HNRNPF antibodies

Anti-HNRNPF TRUEMAB Antibody Clone OTI5F5

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA500804 HNRNPF (hnRNP F) mouse monoclonal antibody, clone OTI5F5 (formerly 5F5) 100ul
30ul
$379 In Stock
LC401544 HNRNPF HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF500804 Carrier-free (BSA/glycerol-free) HNRNPF mouse monoclonal antibody, clone OTI5F5 (formerly 5F5) 100ug $450 3-4 weeks
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WB(2)
IHC(5)
IF(2)
FC(2)
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Also for HNRNPF (NM_004966)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human HNRNPF (NP_004957) produced in HEK293T cell.
Clone NameClone OTI5F5 IsotypeIgG2a
Species ReactivityHuman Concentration0.61 mg/ml
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsWB 1:2000, IHC 1:50, IF 1:50~100, FLOW 1:100
Predicted MW Explanation 45.7 kDa
BufferPBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens heterogeneous nuclear ribonucleoprotein F (HNRNPF), transcript variant 3
Alternative NameHNRPF; mcs94-1; OK/SW-cl.23
Database LinkNP_004957
Entrez Gene 3185 Human
FunctionThis gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins that complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and regulate alternative splicing, polyadenylation, and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has three repeats of quasi-RRM domains that bind to RNAs which have guanosine-rich sequences. This protein is very similar to the family member hnRPH. Multiple alternatively spliced variants, encoding the same protein, have been identified.
Related Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HNRNPF (RC205100, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HNRNPF.
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Western blot analysis of extracts (10ug) from 10 Human tissue by using anti-HNRNPF monoclonal antibody at 1:1000 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).
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Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-HNRNPF mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500804)
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Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-HNRNPF mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500804)
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-HNRNPF mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500804)
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Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-HNRNPF mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500804)
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-HNRNPF mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500804)
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Anti-HNRNPF mouse monoclonal antibody (TA500804) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY HNRNPF(RC205100).
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Immunofluorescent staining of HepG2 cells using anti-HNRNPF mouse monoclonal antibody (TA500804).
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Flow cytometric Analysis of Hela cells, using anti-HNRNPF antibody(TA500804),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
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Flow cytometric Analysis of Jurkat cells, using anti-HNRNPF antibody(TA500804),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
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