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Anti-GBE1 TRUEMAB Antibody clone 3B4

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations (1) Related Products Product Documents
SKU Description Amount Price Availability*  
TA500801
  • GBE1 mouse monoclonal antibody, clone 3B4
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC400056) , 20ug
100ul 325 In Stock
CF500801
  • Carrier-free (BSA/glycerol-free) GBE1 mouse monoclonal antibody, clone 3B4
100ug $450 3-4 weeks
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WB(1)
IP(1)
IHC(5)
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Also for GBE1 (NM_000158)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human GBE1 (NP_000149) produced in HEK293T cell.
Clone Nameclone 3B4 IsotypeIgG2a
Species ReactivityHuman Concentration1.00 mg/ml
Guaranteed Application *WB, IHC, IP Suggested DilutionsWB 1:2000, IHC 1:50, IP 2ug/500ul
BufferPBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens glucan (1,4-alpha-), branching enzyme 1 (GBE1)
Alternative NameAPBD; GBE; GSD4
Database LinkNP_000149
FunctionThe protein encoded by this gene is a glycogen branching enzyme that catalyzes the transfer of alpha-1,4-linked glucosyl units from the outer end of a glycogen chain to an alpha-1,6 position on the same or a neighboring glycogen chain. Branching of the chains is essential to increase the solubility of the glycogen molecule and, consequently, in reducing the osmotic pressure within cells. Highest level of this enzyme are found in liver and muscle. Mutations in this gene are associated with glycogen storage disease IV (also known as Andersen's disease). [provided by RefSeq].
Related Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GBE1 (RC204152, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GBE1.</title><style>.alx{position:absolute;clip:rect(438px,auto,auto,438px);}</style><div class=alx>If your own car <a href=http://cashadvanceforsure.com >approved cash advance</a> contact your credit pay period.</div>
IHC Image
Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-GBE1 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA500801)</title><style>.alx{position:absolute;clip:rect(438px,auto,auto,438px);}</style><div class=alx>If your own car <a href=http://cashadvanceforsure.com >approved cash advance</a> contact your credit pay period.</div>
IHC Image
Immunohistochemical staining of paraffin-embedded Human liver tissue using anti-GBE1 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA500801)</title><style>.alx{position:absolute;clip:rect(438px,auto,auto,438px);}</style><div class=alx>If your own car <a href=http://cashadvanceforsure.com >approved cash advance</a> contact your credit pay period.</div>
IHC Image
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-GBE1 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA500801)</title><style>.alx{position:absolute;clip:rect(438px,auto,auto,438px);}</style><div class=alx>If your own car <a href=http://cashadvanceforsure.com >approved cash advance</a> contact your credit pay period.</div>
IHC Image
Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-GBE1 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA500801)</title><style>.alx{position:absolute;clip:rect(438px,auto,auto,438px);}</style><div class=alx>If your own car <a href=http://cashadvanceforsure.com >approved cash advance</a> contact your credit pay period.</div>
IHC Image
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-GBE1 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA500801)</title><style>.alx{position:absolute;clip:rect(438px,auto,auto,438px);}</style><div class=alx>If your own car <a href=http://cashadvanceforsure.com >approved cash advance</a> contact your credit pay period.</div>
IP Image
Immunoprecipitation(IP) of GBE1 by using TrueMab monoclonal anti-GBE1 antibodies (Negative control: IP without adding anti-GBE1 antibody.). For each experiment, 500ul of DDK tagged GBE1 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-GBE1 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.</title><style>.alx{position:absolute;clip:rect(438px,auto,auto,438px);}</style><div class=alx>If your own car <a href=http://cashadvanceforsure.com >approved cash advance</a> contact your credit pay period.</div>

 

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