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Anti-LEMD3 TRUEMAB Antibody Clone OTI6C10

TrueMAB™ Antibodies - Made against Authentic Protein Antigens


Specifications Citations (1) Customer Reviews Product Documents
SKU Description Amount Price Availability*  
TA500794 anti-LEMD3 (MAN1) mouse monoclonal antibody, clone OTI6C10 (formerly 6C10) 100ul $325 In Stock
LC402311 LEMD3 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF500794 Carrier-free (BSA/glycerol-free) LEMD3 mouse monoclonal antibody, clone OTI6C10 (formerly 6C10) 100ug $450 4 Weeks
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OriGene Data

ImmunogenFull length human recombinant protein of human LEMD3 (NP_055134) produced in HEK293T cell.
Clone NameClone OTI6C10 IsotypeIgG1
Species ReactivityHuman, Dog, Monkey Concentration0.56 mg/ml
Guaranteed Application *WB, IF, IP, FC Suggested DilutionsWB 1:1000, IF 1:100, Flow 1:100, IP 2-4ug/mg
Predicted MW Explanation 100.0 kDa
BufferPBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameLEM domain containing 3
Alternative NameMAN1
Database LinkNP_055134
Entrez Gene 23592 Human
Entrez Gene 717597 Monkey
Entrez Gene 608380 Dog
FunctionThis locus encodes a LEM domain-containing protein. The encoded protein functions to antagonize transforming growth factor-beta signaling at the inner nuclear membrane. Two transcript variants encoding different isoforms have been found for this gene. Mutations in this gene have been associated with osteopoikilosis, Buschke-Ollendorff syndrome and melorheostosis
Related PathwayTransmembrane

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WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LEMD3 (RC212759, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LEMD3.

WB Image
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-LEMD3 monoclonal antibody.

IF Image
Anti-LEMD3 mouse monoclonal antibody (TA500794) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY LEMD3(RC212759).

IP Image
Immunoprecipitation of LEMD3 by using TrueMab monoclonal anti-LEMD3 antibody (Negative control: IP without adding anti-LEMD3 antibody). For each experiment, 500ul of DDK tagged LEMD3 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-LEMD3 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.

FC Image
Flow cytometric analysis of Hela cells, using anti-LEMD3 antibody(TA500794),(Red) compared to a nonspecific negative control antibody(TA50011)(Blue).

FC Image
Flow cytometric analysis of Jurkat cells, using anti-LEMD3 antibody(TA500794),(Red) compared to a nonspecific negative control antibody(TA50011)(Blue).


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