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Home Antibody All anti-TTLL12 antibodies
KO-Validated

Anti-TTLL12 TRUEMAB Antibody Clone OTI5H3

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations Customer Reviews Product Documents
SKU Description Amount Price Availability*  
TA500780 anti-TTLL12 mouse monoclonal antibody, clone OTI5H3 (formerly 5H3) 100ul $325 In Stock
LC402417 TTLL12 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF500780 Carrier-free (BSA/glycerol-free) TTLL12 mouse monoclonal antibody, clone OTI5H3 (formerly 5H3) 100ug $450 4 Weeks
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WB(3)
IHC(7)
IF(1)
FC(1)
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Also for TTLL12 (NM_015140)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human TTLL12 (NP_055955) produced in HEK293T cell.
Clone NameClone OTI5H3 IsotypeIgG1
Species ReactivityHuman, Dog Concentration0.58 mg/ml
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsWB 1:2000, IHC 1:50, IF 1:100, Flow: 1:100
Predicted MW Explanation 74.4 kDa
BufferPBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens tubulin tyrosine ligase like 12 (TTLL12)
Alternative NamedJ526I14.2
Database LinkNP_055955
Entrez Gene 23170 Human
Entrez Gene 474474 Dog
Function
Related Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.


WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TTLL12 (RC200841, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TTLL12.
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WB Image
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-TTLL12 monoclonal antibody.
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WB Image
Equivalent amounts of cell lysates (10 ug per lane) of wild-type 293T cells (WT, Cat# LC810293T) and TTLL12-Knockout 293T cells (KO, Cat# LC811864) were separated by SDS-PAGE and immunoblotted with anti-TTLL12 monoclonal antibody TA500780,(1:500). Then the blotted membrane was stripped and reprobed with anti-b-actin antibody (TA811000) as a loading control.
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IHC Image
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-TTLL12 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500780, Dilution 1:50)
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IHC Image
Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-TTLL12 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500780, Dilution 1:50)
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IHC Image
Immunohistochemical staining of paraffin-embedded liver tissue within the normal limits using anti-TTLL12 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500780, Dilution 1:50)
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IHC Image
Immunohistochemical staining of paraffin-embedded Carcinoma of liver tissue using anti-TTLL12 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500780, Dilution 1:50)
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IHC Image
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-TTLL12 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500780, Dilution 1:50)
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IHC Image
Immunohistochemical staining of paraffin-embedded prostate tissue within the normal limits using anti-TTLL12 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500780, Dilution 1:50)
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IHC Image
Immunohistochemical staining of paraffin-embedded Carcinoma of prostate tissue using anti-TTLL12 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500780, Dilution 1:50)
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IF Image
Anti-TTLL12 mouse monoclonal antibody (TA500780) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY TTLL12(RC200841).
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FC Image
HEK293T cells transfected with either RC200841 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-TTLL12 antibody(TA500780), and then analyzed by flow cytometry.
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Non-US customers are encouraged to contact our dedicated distributor network for quotes and streamlined ordering/delivery support.

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