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Home Antibody All anti-IDH3A antibodies

Anti-IDH3A TRUEMAB Antibody Clone OTI2F11

TrueMAB™ Antibodies - Made against Authentic Protein Antigens


Specifications Citations (1) Customer Reviews Product Documents
SKU Description Amount Price Availability*  
TA500739 anti-IDH3A mouse monoclonal antibody, clone OTI2F11 (formerly 2F11) 100ul $379 In Stock
LC401698 IDH3A HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF500739 Carrier-free (BSA/glycerol-free) IDH3A mouse monoclonal antibody, clone OTI2F11 (formerly 2F11) 100ug $450 4 Weeks
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Also for IDH3A (NM_005530)
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OriGene Data

ImmunogenFull length human recombinant protein of human IDH3A (NP_005521) produced in HEK293T cell.
Clone NameClone OTI2F11 IsotypeIgG2a
Species ReactivityHuman, Dog Concentration1.00mg/ml
Guaranteed Application *WB, IF, FC Suggested DilutionsWB 1:2000, IF 1:100, Flow 1:100
Predicted MW Explanation 39.6 kDa
BufferPBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from cell culture supernatant by affinity chromatography

Reference Data

Target Nameisocitrate dehydrogenase 3 (NAD(+)) alpha
Alternative Name-
Database LinkNP_005521
Entrez Gene 3419 Human
Entrez Gene 479066 Dog
FunctionIsocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. NAD(+)-dependent isocitrate dehydrogenases catalyze the allosterically regulated rate-limiting step of the tricarboxylic acid cycle. Each isozyme is a heterotetramer that is composed of two alpha subunits, one beta subunit, and one gamma subunit. The protein encoded by this gene is the alpha subunit of one isozyme of NAD(+)-dependent isocitrate dehydrogenase.
Related Pathway Citrate cycle (TCA cycle)Metabolic pathways

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IDH3A (RC200313, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IDH3A.

WB Image
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-IDH3A monoclonal antibody.

WB Image
Figure from citation: Western blot analysis of IDH3A protein level by using anti-IDH3A antibody in fibroblasts with miR-424 knockdown or overexpression,and in TGF-b1-treated fibroblasts with or without miR-424 depletion. View Citation

IF Image
Anti-IDH3A mouse monoclonal antibody (TA500739) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY IDH3A(RC200313).

IF Image
Immunofluorescent staining of HepG2 cells using anti-IDH3A mouse monoclonal antibody (TA500739).

FC Image
Flow cytometric analysis of Hela cells, using anti-IDH3A antibody(TA500739),(Red) compared to a nonspecific negative control antibody(TA50011)(Blue).

FC Image
Flow cytometric analysis of Jurkat cells, using anti-IDH3A antibody(TA500739),(Red) compared to a nonspecific negative control antibody(TA50011)(Blue).


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