OriGene Logo
Search:    
Left ProductsProducts divider ServicesServices divider technologyTechnology divider researchResearch divider TechsupportTechSupport divider AboutAbout Right
 
Home Antibody All anti-L1CAM antibodies

Anti-L1CAM TRUEMAB Antibody Clone OTI2A6

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

div

Specifications Citations Customer Reviews Product Documents
SKU Description Amount Price Availability*  
TA500728 anti-L1CAM mouse monoclonal antibody, clone OTI2A6 (formerly 2A6) 100ul $325 In Stock
LC400150 L1CAM HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF500728 Carrier-free (BSA/glycerol-free) L1CAM mouse monoclonal antibody, clone OTI2A6 (formerly 2A6) 100ug $450 4 Weeks
Add to Shopping Cart
spacer
WB(2)
IP(1)
IHC(8)
IF(1)
FC(2)
spacer
Also for L1CAM (NM_000425)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human IDH3A (NP_005521) produced in HEK293T cell.
Clone NameClone OTI2A6 IsotypeIgG1
Species ReactivityHuman Concentration0.97 mg/ml
Guaranteed Application *WB, IHC, IF, IP, FC Suggested DilutionsWB 1:1000, IHC 1:50, IF 1:100, Flow 1:100, IP 2-4ug/mg
Predicted MW Explanation 140.0 kDa
BufferPBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameL1 cell adhesion molecule
Alternative NameCAML1|CD171|HSAS|HSAS1|MASA|MIC5|N-CAM-L1|N-CAML1|NCAM-L1|S10|SPG1
Database LinkNP_000416
Entrez Gene 3897 Human
FunctionThe protein encoded by this gene is an axonal glycoprotein belonging to the immunoglobulin supergene family. The ectodomain, consisting of several immunoglobulin-like domains and fibronectin-like repeats (type III), is linked via a single transmembrane sequence to a conserved cytoplasmic domain. This cell adhesion molecule plays an important role in nervous system development, including neuronal migration and differentiation. Mutations in the gene cause three X-linked neurological syndromes known by the acronym CRASH (corpus callosum hypoplasia, retardation, aphasia, spastic paraplegia and hydrocephalus). Alternative splicing of a neuron-specific exon is thought to be functionally relevant.
Related PathwayES Cell Differentiation/IPSTransmembraneDruggable Genome Axon guidanceCell adhesion molecules (CAMs)

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.


WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY L1CAM (RC211601, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-L1CAM.
grey_line

WB Image
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-L1CAM monoclonal antibody.
grey_line

IHC Image
Immunohistochemical staining of paraffin-embedded breast tissue within the normal limits using anti-L1CAM mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500728, Dilution 1:50)
grey_line

IHC Image
Immunohistochemical staining of paraffin-embedded colon tissue within the normal limits using anti-L1CAMmouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500728, Dilution 1:50)
grey_line

IHC Image
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-L1CAMmouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500728, Dilution 1:50)
grey_line

IHC Image
Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-L1CAMmouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500728, Dilution 1:50)
grey_line

IHC Image
Immunohistochemical staining of paraffin-embedded Ovary tissue within the normal limits using anti-L1CAMmouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500728, Dilution 1:50)
grey_line

IHC Image
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-L1CAMmouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500728, Dilution 1:50)
grey_line

IHC Image
Immunohistochemical staining of paraffin-embedded pancreas tissue within the normal limits using anti-L1CAMmouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500728, Dilution 1:50)
grey_line

IHC Image
Immunohistochemical staining of paraffin-embedded endometrium tissue within the normal limits using anti-L1CAMmouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500728, Dilution 1:50)
grey_line

IF Image
Anti-L1CAM mouse monoclonal antibody (TA500728) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY L1CAM(RC211601).
grey_line

IP Image
Immunoprecipitation of L1CAM by using TrueMab monoclonal anti-L1CAM antibody (Negative control: IP without adding anti-L1CAM antibody). For each experiment, 500ul of DDK tagged L1CAM overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-L1CAM antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
grey_line

FC Image
Flow cytometric analysis of Hela cells, using anti-L1CAM antibody(TA500728),(Red) compared to a nonspecific negative control antibody(TA50011)(Blue).
grey_line

FC Image
Flow cytometric analysis of Jurkat cells, using anti-L1CAM antibody(TA500728),(Red) compared to a nonspecific negative control antibody(TA50011)(Blue).
grey_line

 

spacer
Inc 5000 Healthcare Company

Notice to Non-US Customers

ExclaimationWeb price and delivery time are for direct sales only.

Non-US customers are encouraged to contact our dedicated distributor network for quotes and streamlined ordering/delivery support.

Acknowledge