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Anti-CYP2E1 TRUEMAB Antibody Clone OTI5F11
TrueMAB Antibodies - Made against Authentic Protein Antigens
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Also for CYP2E1 (NM_000773)
|Full length human recombinant protein of human CYP2E1 (NP_000764) produced in HEK293T cell.|
|WB, IHC, IF
||WB 1:500, IHC 1:50, IF 1:100,
|PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
|Homo sapiens cytochrome P450, family 2, subfamily E, polypeptide 1 (CYP2E1)|
|CPE1; CYP2E; P450-J; P450C2E|
Entrez Gene 1571 Human
|This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and is induced by ethanol, the diabetic state, and starvation. The enzyme metabolizes both endogenous substrates, such as ethanol, acetone, and acetal, as well as exogenous substrates including benzene, carbon tetrachloride, ethylene glycol, and nitrosamines which are premutagens found in cigarette smoke. Due to its many substrates, this enzyme may be involved in such varied processes as gluconeogenesis, hepatic cirrhosis, diabetes, and cancer.|
|TransmembraneP450Druggable Genome Arachidonic acid metabolismLinoleic acid metabolismMetabolism of xenobiotics by cytochrome P450Drug metabolism - cytochrome P450Metabolic pathways|
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CYP2E1 (RC213502, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CYP2E1 .
Immunohistochemical staining of paraffin-embedded liver tissue within the normal limits using anti-CYP2E1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500717, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded Ovary tissue within the normal limits using anti-CYP2E1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500717, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded endometrium tissue within the normal limits using anti-CYP2E1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500717, Dilution 1:50)
Immunofluorescent staining of HepG2 cells using anti-CYP2E1 mouse monoclonal antibody (TA500717).