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Anti-ID3 TRUEMAB Antibody clone 15F4

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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SKU Description Amount Price Availability*  
TA500716
  • ID3 mouse monoclonal antibody, clone 15F4
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC419492) , 20ug Explanation
100ul $379 In Stock
CF500716
  • Carrier-free (BSA/glycerol-free) ID3 mouse monoclonal antibody, clone 15F4
100ug $450 3-4 weeks
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WB(2)
IHC(14)
IF(1)
FC(2)

OriGene Data

ImmunogenFull length human recombinant protein of human ID3 (NP_002158) produced in HEK293T cell.
Clone Nameclone 15F4 IsotypeIgG1
Species ReactivityHuman Concentration0.59 mg/ml
Guaranteed Application *WB, IHC, IF, FC Suggested Dilutions IHC 1:100~400, WB: 1:200
Predicted MW Explanation 12.8 kDa
BufferPBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens inhibitor of DNA binding 3, dominant negative helix-loop-helix protein (ID3)
Alternative NamebHLHb25; HEIR-1
Database LinkNP_002158
Entrez Gene 3399 Human
FunctionMembers of the ID family of helix-loop-helix (HLH) proteins lack a basic DNA-binding domain and inhibit transcription through formation of nonfunctional dimers that are incapable of binding to DNA.[supplied by OMIM].
Related PathwayES Cell Differentiation/IPSTranscription FactorsDruggable Genome TGF-beta signaling pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ID3 (RC200583, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ID3.
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Western blot analysis of extracts (10ug) from 3 different cell lines by using anti-ID3 monoclonal antibody.(1:200)
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Immunohistochemical staining of paraffin-embedded Human breast tissue using anti-ID3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500716)
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-ID3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500716)
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Immunohistochemical staining of paraffin-embedded Human colon tissue using anti-ID3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500716)
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-ID3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500716)
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Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-ID3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500716)
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Immunohistochemical staining of paraffin-embedded Human liver tissue using anti-ID3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500716)
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Immunohistochemical staining of paraffin-embedded Human lung tissue using anti-ID3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500716)
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Immunohistochemical staining of paraffin-embedded Human Ovary tissue using anti-ID3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500716)
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-ID3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500716)
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Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-ID3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500716)
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Immunohistochemical staining of paraffin-embedded Human thyroid tissue using anti-ID3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500716)
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Immunohistochemical staining of paraffin-embedded Human endometrium tissue using anti-ID3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500716)
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Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-ID3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500716)
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Immunohistochemical staining of paraffin-embedded Human bladder tissue using anti-ID3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500716)
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Anti-ID3 mouse monoclonal antibody (TA500716) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ID3(RC200583).
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Flow cytometric Analysis of Hela cells, using anti-ID3 antibody(TA500716),(Red), compared to a nonspecific negative control antibody,(Blue).
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Flow cytometric Analysis of Jurkat cells, using anti-ID3 antibody(TA500716),(Red), compared to a nonspecific negative control antibody,(Blue).
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