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Home Antibody All anti-BUB1B antibodies

Anti-BUB1B TRUEMAB Antibody Clone OTI6E3

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations Customer Reviews Product Documents
SKU Description Amount Price Availability*  
TA500679 BuB1B (BubR1) mouse monoclonal antibody, clone OTI6E3 (formerly 6E3) 100ul $379 In Stock
LC420068 BUB1B HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF500679 Carrier-free (BSA/glycerol-free) BuB1B mouse monoclonal antibody, clone OTI6E3 (formerly 6E3) 100ug $450 4 Weeks
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WB(2)
IF(1)
FC(3)
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Also for BUB1B (NM_001211)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human BUB1B (NP_001202) produced in HEK293T cell.
Clone NameClone OTI6E3 IsotypeIgG1
Species ReactivityHuman, Dog, Rat, Monkey Concentration1.00mg/ml
Guaranteed Application *IF, WB, FC Suggested DilutionsWB 1:500~2000, IF 1:100, FLOW 1:100
Predicted MW Explanation 119.3 kDa
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from cell culture supernatant by affinity chromatography

Reference Data

Target NameBUB1 mitotic checkpoint serine/threonine kinase B
Alternative NameBUB1beta|BUBR1|Bub1A|MAD3L|MVA1|SSK1|hBUBR1
Database LinkNP_001202
Entrez Gene 701 Human
Entrez Gene 171576 Rat
Entrez Gene 100423079 Monkey
Entrez Gene 608350 Dog
FunctionThis gene encodes a kinase involved in spindle checkpoint function. The protein has been localized to the kinetochore and plays a role in the inhibition of the anaphase-promoting complex/cyclosome (APC/C), delaying the onset of anaphase and ensuring proper chromosome segregation. Impaired spindle checkpoint function has been found in many forms of cancer. [provided by RefSeq].
Related PathwayProtein KinaseStem cell - PluripotencyDruggable Genome Cell cycle

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.


IF Image
Anti-BUB1B mouse monoclonal antibody (TA500679) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BUB1B(RC205844).
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WB Image
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-BUB1B monoclonal antibody.
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WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BUB1B (RC205844, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BUB1B.
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FC Image
Flow cytometric Analysis of Jurkat cells, using anti-BUB1B antibody(TA500679),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
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FC Image
Flow cytometric Analysis of Hela cells, using anti-BUB1B antibody(TA500679),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
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FC Image
HEK293T cells transfected with either RC205844 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-BUB1B antibody(TA500679), and then analyzed by flow cytometry.
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