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Anti-BUB1B TrueMAB Antibody clone 6E3
TrueMAB Antibodies - Made against Authentic Protein Antigens
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Also for BUB1B (NM_001211)
|Full length human recombinant protein of human BUB1B (NP_001202) produced in HEK293T cell.|
|Human, Dog, Rat, Monkey
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IF, FC
||WB 1:500~2000, IF 1:100, FLOW 1:100
|PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography (Protein A or G Sepharose)
|Homo sapiens BUB1 mitotic checkpoint serine/threonine kinase B (BUB1B)|
|Bub1A; BUB1beta; BUBR1; hBUBR1; MAD3L; MVA1; SSK1|
|This gene encodes a kinase involved in spindle checkpoint function. The protein has been localized to the kinetochore and plays a role in the inhibition of the anaphase-promoting complex/cyclosome (APC/C), delaying the onset of anaphase and ensuring proper chromosome segregation. Impaired spindle checkpoint function has been found in many forms of cancer. [provided by RefSeq].|
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BUB1B (RC205844, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BUB1B.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-BUB1B monoclonal antibody.
Anti-BUB1B mouse monoclonal antibody (TA500679) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BUB1B(RC205844).
HEK293T cells transfected with either RC205844 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-BUB1B antibody(TA500679), and then analyzed by flow cytometry.
Flow cytometric Analysis of Hela cells, using anti-BUB1B antibody(TA500679),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
Flow cytometric Analysis of Jurkat cells, using anti-BUB1B antibody(TA500679),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).