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Anti-MAP2K1 TRUEMAB Antibody clone 1C1
TrueMAB Antibodies - Made against Authentic Protein Antigens
Also for MAP2K1 (NM_002755)
|Full-length protein expressed in 293T cell transfected with human MAP2K1 expression vector|
|WB, IHC, IF
||WB 1:2000, IHC 1:50, IF 1:100,
|PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
|Homo sapiens mitogen-activated protein kinase kinase 1 (MAP2K1)|
|CFC3; MAPKK1; MEK1; MKK1; PRKMK1|
|The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development. |
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAP2K1 (RC218460, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAP2K1 .
Immunohistochemical staining of paraffin-embedded colon tissue using anti-MAP2K1 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA500672, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded endometrium tissue using anti-MAP2K1 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA500672, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded prostate tissue using anti-MAP2K1 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA500672, Dilution 1:50)
Anti-MAP2K1 mouse monoclonal antibody (TA500672) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MAP2K1 (RC218460).
Immunofluorescent staining of HT29 cells using anti-MAP2K1 mouse monoclonal antibody (TA500672).