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Anti-FCGR2A TRUEMAB Antibody Clone OTI6D11
TrueMAB Antibodies - Made against Authentic Protein Antigens
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Also for FCGR2A (NM_021642)
|Full length human recombinant protein of human FCGR2A (NP_067674) produced in HEK293T cell.|
|WB, IHC, IP, FC
||WB 1:200 - 1:1000, IHC 1:50, FLOW 1:100, IP 2ug/500ul
|PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
|Homo sapiens Fc fragment of IgG receptor IIa (FCGR2A), transcript variant 2|
|CD32; CD32A; CDw32; FCG2; FcGR; FCGR2; FCGR2A1; IGFR2|
Entrez Gene 2212 Human
|This gene encodes one member of a family of immunoglobulin Fc receptor genes found on the surface of many immune response cells. The protein encoded by this gene is a cell surface receptor found on phagocytic cells such as macrophages and neutrophils, and is involved in the process of phagocytosis and clearing of immune complexes. Alternative splicing results in multiple transcript variants. [provided by RefSeq].|
|ES Cell Differentiation/IPSTransmembrane Fc gamma R-mediated phagocytosisSystemic lupus erythematosus|
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FCGR2A (RC205786, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FCGR2A.
Western blot analysis of extracts (10ug) from 1 cell line by using anti-FCGR2A monoclonal antibody at 1:200.
Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-FCGR2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500653)
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-FCGR2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500653)
Immunoprecipitation(IP) of FCGR2A by using TrueMab monoclonal anti-FCGR2A antibodies (Negative control: IP without adding anti-FCGR2A antibody.). For each experiment, 500ul of DDK tagged FCGR2A overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-FCGR2A antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
HEK293T cells transfected with either RC205786 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-FCGR2A antibody(TA500653), and then analyzed by flow cytometry.