Anti-FCGR2A Antibody, clone 19C10

Also for FCGR2A (NM_021642)

Gene Name	Homo sapiens Fc fragment of IgG, low affinity IIa, receptor (CD32) (FCGR2A), transcript variant 2
Synonyms:	CD32; CD32A; CDw32; FCG2; FcGR; FCGR2; FCGR2A1; IGFR2
Immunogen	Full length human recombinant protein of human FCGR2A (NP_067674) produced in HEK293T cell.
Buffer	PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Clone Name	clone 19C10	Isotype	IgG1
Species Reactivity	Human	Concentration	0.5~1.0 mg/ml (Lot Dependent)
Purification	Purified from mouse ascites fluids by affinity chromatography (Protein A or G Sepharose)
Guaranteed Application *	WB, IHC, IF, IP, FC	Suggested Dilutions	WB 1:2000, IHC 1:50, IF 1:100, FLOW 1:100, IP 2ug/500ul
Background	This gene encodes one member of a family of immunoglobulin Fc receptor genes found on the surface of many immune response cells. The protein encoded by this gene is a cell surface receptor found on phagocytic cells such as macrophages and neutrophils, and is involved in the process of phagocytosis and clearing of immune complexes. Alternative splicing results in multiple transcript variants. [provided by RefSeq].
Related Pathway

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WB Image

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FCGR2A (RC205786, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FCGR2A.

IHC Image

Immunohistochemical staining of paraffin-embedded Human colon tissue using anti-FCGR2A mouse monoclonal antibody. (TA500647)

IHC Image

Immunohistochemical staining of paraffin-embedded Human Ovary tissue using anti-FCGR2A mouse monoclonal antibody. (TA500647)

IHC Image

Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-FCGR2A mouse monoclonal antibody. (TA500647)

IHC Image

Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-FCGR2A mouse monoclonal antibody. (TA500647)

IHC Image

Immunohistochemical staining of paraffin-embedded Human endometrium tissue using anti-FCGR2A mouse monoclonal antibody. (TA500647)

IHC Image

Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-FCGR2A mouse monoclonal antibody. (TA500647)

IF Image

Anti-FCGR2A mouse monoclonal antibody (TA500647) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY FCGR2A(RC205786).

IP Image

Immunoprecipitation(IP) of FCGR2A by using TrueMab monoclonal anti-FCGR2A antibodies (Negative control: IP without adding anti-FCGR2A antibody.). For each experiment, 500ul of DDK tagged FCGR2A overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-FCGR2A antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.

FC Image

HEK293T cells transfected with either RC205786 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-FCGR2A antibody(TA500647), and then analyzed by flow cytometry.

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Anti-FCGR2A TrueMAB Antibody clone 19C10

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