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Home Antibody All anti-PRKAR1A antibodies

Anti-PRKAR1A TRUEMAB Antibody Clone OTI6C7

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations (2) Related Products Product Documents
SKU Description Amount Price Availability*  
TA500641 anti-PRKAR1A (PKA regulatory subunit I alpha) mouse monoclonal antibody, clone OTI6C7 (formerly 6C7) 100ul $379 In Stock
LC403945 PRKAR1A HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF500641 Carrier-free (BSA/glycerol-free) PRKAR1A mouse monoclonal antibody, clone OTI6C7 (formerly 6C7) 100ug $450 3 Days
Conjugation is available for this antibody: choose conjugation type
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WB(2)
IHC(7)
IF(1)
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Also for PRKAR1A (NM_212472)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenFull-length protein expressed in 293T cell transfected with human PRKAR1A expression vector
Clone NameClone OTI6C7 IsotypeIgG1
Species ReactivityHuman Concentration1 mg/ml
Guaranteed Application *WB, IHC, IF Suggested DilutionsWB 1:2000, IHC 1:50, IF 1:100,
Predicted MW Explanation 43.0 KDa
BufferPBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens protein kinase, cAMP-dependent, regulatory, type I, alpha (PRKAR1A), transcript variant 3
Alternative NameACRDYS1; ADOHR; CAR; CNC; CNC1; PKR1; PPNAD1; PRKAR1; TSE1
Database LinkNP_997637
Entrez Gene 5573 Human
FunctioncAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. This gene encodes one of the regulatory subunits. This protein was found to be a tissue-specific extinguisher that down-regulates the expression of seven liver genes in hepatoma x fibroblast hybrids. Mutations in this gene cause Carney complex (CNC). This gene can fuse to the RET protooncogene by gene rearrangement and form the thyroid tumor-specific chimeric oncogene known as PTC2. A nonconventional nuclear localization sequence (NLS) has been found for this protein which suggests a role in DNA replication via the protein serving as a nuclear transport protein for the second subunit of the Replication Factor C (RFC40). Three alternatively spliced transcript variants encoding the same protein have been observed.
Related PathwayTranscription FactorsDruggable Genome ApoptosisInsulin signaling pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PRKAR1A (RC212810, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PRKAR1A.
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PRKAR1A monoclonal antibody.
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-PRKAR1A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500641, Dilution 1:50)
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Immunohistochemical staining of paraffin-embedded colon tissue within the normal limits using anti-PRKAR1Amouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500641, Dilution 1:50)
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Immunohistochemical staining of paraffin-embedded Ovary tissue within the normal limits using anti-PRKAR1Amouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500641, Dilution 1:50)
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Immunohistochemical staining of paraffin-embedded endometrium tissue within the normal limits using anti-PRKAR1Amouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500641, Dilution 1:50)
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Immunohistochemical staining of paraffin-embedded prostate tissue within the normal limits using anti-PRKAR1Amouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500641, Dilution 1:50)
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Immunohistochemical staining of paraffin-embedded Carcinoma of bladder tissue using anti-PRKAR1Amouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500641, Dilution 1:50)
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Figure from citation: PRKAR1A IHC in a CNC-associated cardiac myxoma. A, A photomicrograph of the specimen at low power exhibits the robust reactivity of the adjacent normal myocardium with antibodies directed against PRKAR1A. B, A high-power photomicrograph exhibits the lack of reactivity seen in the neoplastic (myxoma) cells, although the intratumoral histiocytes are still strongly reactive. Dilution: 1:8000 View Citation
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Anti-PRKAR1A mouse monoclonal antibody (TA500641) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PRKAR1A(RC212810).
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