Anti-BUB1B Antibody, clone 3D9

Also for BUB1B (NM_001211)

OriGene Data

Immunogen	Protein expressed in 293T cell transfected with human BUB1B expression vector
Clone Name	clone 3D9	Isotype	IgG2b
Species Reactivity	Human	Concentration	0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *	WB, IHC, IF, FC	Suggested Dilutions	WB 1:2000, IHC 1:50, IF 1:100, Flow 1:100
Buffer	PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification	Purified from mouse ascites fluids by affinity chromatography (Protein A or G Sepharose)

Reference Data

Target Name	Homo sapiens BUB1 mitotic checkpoint serine/threonine kinase B (BUB1B)
Alternative Name	Bub1A; BUB1beta; BUBR1; hBUBR1; MAD3L; MVA1; SSK1
Database Link	NP_001202
Function	This gene encodes a kinase involved in spindle checkpoint function. The protein has been localized to the kinetochore and plays a role in the inhibition of the anaphase-promoting complex/cyclosome(APC/C), delaying the onset of anaphase and ensuring proper chromosome segregation. Impaired spindle checkpoint function has been found in many forms of cancer.
Related Pathway	Cell cycle

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WB Image

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BUB1B (RC205844, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BUB1B.

WB Image

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-BUB1B monoclonal antibody.

IHC Image

Immunohistochemical staining of paraffin-embedded colon tissue using anti-BUB1Bmouse monoclonal antibody. (TA500609, Dilution 1:50)

IHC Image

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-BUB1Bmouse monoclonal antibody. (TA500609, Dilution 1:50)

IHC Image

Immunohistochemical staining of paraffin-embedded Ovary tissue using anti-BUB1Bmouse monoclonal antibody. (TA500609, Dilution 1:50)

IHC Image

Immunohistochemical staining of paraffin-embedded pancreas tissue using anti-BUB1Bmouse monoclonal antibody. (TA500609, Dilution 1:50)

IHC Image

Immunohistochemical staining of paraffin-embedded endometrium tissue using anti-BUB1Bmouse monoclonal antibody. (TA500609, Dilution 1:50)

IHC Image

Immunohistochemical staining of paraffin-embedded prostate tissue using anti-BUB1Bmouse monoclonal antibody. (TA500609, Dilution 1:50)

IHC Image

Immunohistochemical staining of paraffin-embedded Carcinoma of bladder tissue using anti-BUB1Bmouse monoclonal antibody. (TA500609, Dilution 1:50)

IF Image

Immunofluorescent staining of HeLa cells using anti-BUB1B mouse monoclonal antibody (TA500609).

FC Image

HEK293T cells transfected with either pCMV6-ENTRY BUB1B(RC205844)(Red) or empty vector control plasmid(Blue) were immunostained with anti-BUB1B mouse monoclonal(TA500609, Dilution 1:1,000), and then analyzed by flow cytometry.

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Anti-BUB1B TrueMAB Antibody clone 3D9

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

OriGene Data

Reference Data