This gene encodes a kinase involved in spindle checkpoint function. The protein has been localized to the kinetochore and plays a role in the inhibition of the anaphase-promoting complex/cyclosome(APC/C), delaying the onset of anaphase and ensuring proper chromosome segregation. Impaired spindle checkpoint function has been found in many forms of cancer.
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BUB1B (RC205844, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BUB1B.
HEK293T cells transfected with either pCMV6-ENTRY BUB1B(RC205844)(Red) or empty vector control plasmid(Blue) were immunostained with anti-BUB1B mouse monoclonal(TA500607, Dilution 1:1,000), and then analyzed by flow cytometry.
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