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Home Antibody All anti-BUB1B antibodies

Anti-BUB1B TRUEMAB Antibody Clone OTI6E5

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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SKU Description Amount Price Availability*  
TA500607 anti-BUB1B (BubR1) mouse monoclonal antibody, clone OTI6E5 (formerly 6E5) 100ul $325 In Stock
LC420068 BUB1B HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF500607 Carrier-free (BSA/glycerol-free) BUB1B mouse monoclonal antibody, clone OTI6E5 (formerly 6E5) 100ug $450 3-4 weeks
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WB(2)
IHC(7)
IF(2)
FC(1)
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Also for BUB1B (NM_001211)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenFull-length protein expressed in 293T cell transfected with human BUB1B expression vector
Clone NameClone OTI6E5 IsotypeIgG1
Species ReactivityHuman Concentration1 mg/ml
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsWB: 1:200 - 1:1000, IHC 1:50, IF 1:100, Flow 1:100
Predicted MW Explanation 119.5 KDa
BufferPBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens BUB1 mitotic checkpoint serine/threonine kinase B (BUB1B)
Alternative NameBub1A; BUB1beta; BUBR1; hBUBR1; MAD3L; MVA1; SSK1
Database LinkNP_001202
Entrez Gene 701 Human
FunctionThis gene encodes a kinase involved in spindle checkpoint function. The protein has been localized to the kinetochore and plays a role in the inhibition of the anaphase-promoting complex/cyclosome(APC/C), delaying the onset of anaphase and ensuring proper chromosome segregation. Impaired spindle checkpoint function has been found in many forms of cancer.
Related PathwayProtein KinaseStem cell - PluripotencyDruggable Genome Cell cycle

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BUB1B (RC205844, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BUB1B.
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Western blot analysis of extracts (10ug) from 5 different cell lines by using anti-BUB1B monoclonal antibody.(1:200)
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-BUB1B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500607, Dilution 1:50)
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Immunohistochemical staining of paraffin-embedded colon tissue within the normal limits using anti-BUB1Bmouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500607, Dilution 1:50)
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-BUB1Bmouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500607, Dilution 1:50)
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Immunohistochemical staining of paraffin-embedded pancreas tissue within the normal limits using anti-BUB1Bmouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500607, Dilution 1:50)
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Immunohistochemical staining of paraffin-embedded endometrium tissue within the normal limits using anti-BUB1Bmouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500607, Dilution 1:50)
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Immunohistochemical staining of paraffin-embedded prostate tissue within the normal limits using anti-BUB1Bmouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500607, Dilution 1:50)
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Immunohistochemical staining of paraffin-embedded Carcinoma of bladder tissue using anti-BUB1Bmouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500607, Dilution 1:50)
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Anti-BUB1B mouse monoclonal antibody (TA500607) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BUB1B(RC205844).
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Immunofluorescent staining of HeLa cells using anti-BUB1B mouse monoclonal antibody (TA500607).
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HEK293T cells transfected with either pCMV6-ENTRY BUB1B(RC205844)(Red) or empty vector control plasmid(Blue) were immunostained with anti-BUB1B mouse monoclonal(TA500607, Dilution 1:1,000), and then analyzed by flow cytometry.
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