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Home Antibody All anti-SLC2A5 antibodies

Anti-SLC2A5 TRUEMAB Antibody Clone OTI12F2

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA500575 SLC2A5 (GLUT5) mouse monoclonal antibody, clone OTI12F2 (formerly 12F2) 100ul
30ul
$325 In Stock
LC401061 SLC2A5 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF500575 Carrier-free (BSA/glycerol-free) SLC2A5 mouse monoclonal antibody, clone OTI12F2 (formerly 12F2) 100ug $450 3 Days
Conjugation is available for this antibody: choose conjugation type
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WB(1)
IP(1)
IF(1)
FC(1)
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Also for SLC2A5 (NM_003039)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human SLC2A5 (NP_003030) produced in HEK293T cell.
Clone NameClone OTI12F2 IsotypeIgG1
Species ReactivityHuman Concentration0.6 mg/ml
Guaranteed Application *WB, IF, IP, FC Suggested DilutionsWB 1:1000, IF 1:50, FLOW 1:100, IP 2ug/500ul
Predicted MW Explanation 54.8 kDa
BufferPBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens solute carrier family 2 (facilitated glucose/fructose transporter), member 5 (SLC2A5), transcript variant 1
Alternative NameGLUT-5; GLUT5
Database LinkNP_003030
Entrez Gene 6518 Human
Function
Related PathwayTransmembrane

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SLC2A5 (RC200418, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SLC2A5.
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Immunofluorescent staining of HT29 cells using anti-SLC2A5 mouse monoclonal antibody (TA500575).
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Immunoprecipitation(IP) of SLC2A5 by using TrueMab monoclonal anti-SLC2A5 antibodies (Negative control: IP without adding anti-SLC2A5 antibody.). For each experiment, 500ul of DDK tagged SLC2A5 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-SLC2A5 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
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HEK293T cells transfected with either RC200418 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-SLC2A5 antibody(TA500575), and then analyzed by flow cytometry.
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