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Anti-POR TRUEMAB Antibody clone 3H10
TrueMAB Antibodies - Made against Authentic Protein Antigens
|Full-length protein expressed in 293T cell transfected with human POR expression vector|
|WB, IHC, IF, FC
||WB 1:1000, IHC 1:50, IF 1:100, Flow 1:100
|PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
|Homo sapiens P450 (cytochrome) oxidoreductase (POR)|
|CPR; CYPOR; P450R|
|This gene encodes an endoplasmic reticulum membrane oxidoreductase with an FAD-binding domain and a flavodoxin-like domain. The protein binds two cofactors, FAD and FMN, which allow it to donate electrons directly from NADPH to all microsomal P450 enzymes. Mutations in this gene have been associated with various diseases, including apparent combined P450C17 and P450C21 deficiency, amenorrhea and disordered steroidogenesis, congenital adrenal hyperplasia and Antley-Bixler syndrome.|
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY POR (RC202172, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-POR.
Immunohistochemical staining of paraffin-embedded pancreas tissue using anti-PORmouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA500572, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded endometrium tissue using anti-PORmouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA500572, Dilution 1:50)
Anti-POR mouse monoclonal antibody (TA500572) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY POR(RC202172).
HEK293T cells transfected with either pCMV6-ENTRY POR(RC202172)(Red) or empty vector control plasmid(Blue) were immunostained with anti-POR mouse monoclonal(TA500572, Dilution 1:1,000), and then analyzed by flow cytometry.