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Home Antibody All anti-DHFR antibodies

Anti-DHFR TRUEMAB Antibody Clone OTI6G7

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA500543 anti-DHFR (Dihydrofolate reductase) mouse monoclonal antibody, clone OTI6G7 (formerly 6G7) 100ul $379 In Stock
LC400271 DHFR HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF500543 Carrier-free (BSA/glycerol-free) DHFR mouse monoclonal antibody, clone OTI6G7 (formerly 6G7) 100ug $450 3-4 weeks
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WB(2)
IHC(4)
IF(1)

OriGene Data

ImmunogenFull-length protein expressed in 293T cell transfected with human DHFR expression vector
Clone NameClone OTI6G7 IsotypeIgG1
Species ReactivityHuman, Monkey, Dog Concentration1.2 mg/ml
Guaranteed Application *WB, IHC, IF Suggested DilutionsWB 1:2000 IHC 1:50 IF 1:100
Predicted MW Explanation 21.5 kDa
BufferPBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens dihydrofolate reductase (DHFR), transcript variant 1
Alternative NameDHFRP1; DYR
Database LinkNP_000782
Entrez Gene 1719 Human
Entrez Gene 711268 Monkey
Entrez Gene 609048 Dog
FunctionDihydrofolate reductase converts dihydrofolate into tetrahydrofolate, a methyl group shuttle required for the de novo synthesis of purines, thymidylic acid, and certain amino acids. While the functional dihydrofolate reductase gene has been mapped to chromosome 5, multiple intronless processed pseudogenes or dihydrofolate reductase-like genes have been identified on separate chromosomes. Dihydrofolate reductase deficiency has been linked to megaloblastic anemia.
Related PathwayStem cell - PluripotencyDruggable Genome One carbon pool by folateFolate biosynthesisMetabolic pathways

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DHFR (RC200089, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DHFR.
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-DHFR monoclonal antibody.
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-DHFR mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500543, Dilution 1:50)
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Immunohistochemical staining of paraffin-embedded Carcinoma of kidney tissue using anti-DHFRmouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500543, Dilution 1:50)
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-DHFRmouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500543, Dilution 1:50)
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Immunohistochemical staining of paraffin-embedded Carcinoma of thyroid tissue using anti-DHFRmouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500543, Dilution 1:50)
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Anti-DHFR mouse monoclonal antibody (TA500543) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY DHFR(RC200089).
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