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BUB1B (Gene ID 701) Antibody

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Catalog No.	Description	Amount	Price	Shipping *
TA500541	Purified anti-BUB1B mouse monoclonal antibody, clone 11D7	100 ul	$325 *	Next Day
TA500541	HEK293T cell transient overexpression lysate (LY420068) as a positive control.	20 ug	Included for FREE

WB(2)

IHC(4)

IF(1)

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Also for BUB1B (NM_001211)

Gene Name	Homo sapiens budding uninhibited by benzimidazoles 1 homolog beta (yeast) (BUB1B)
Synonyms:	Bub1A; BUB1beta; BUBR1; hBUBR1; MAD3L; SSK1
Immunogen	Protein expressed in 293T cell transfected with human BUB1B expression vector
Buffer	PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Clone Name	clone 11D7	Isotype	IgG1
Species Reactivity	Human, Monkey	Concentration	0.5~1.0 mg/ml (Lot Dependent)
Purification	Purified from mouse ascites fluids by affinity chromatography
Guaranteed Application *	WB, IHC, IF	Suggested Dilutions	WB 1:1000 IHC 1:50 IF 1:100
Background	This gene encodes a kinase involved in spindle checkpoint function. The protein has been localized to the kinetochore and plays a role in the inhibition of the anaphase-promoting complex/cyclosome(APC/C), delaying the onset of anaphase and ensuring proper chromosome segregation. Impaired spindle checkpoint function has been found in many forms of cancer.
Related Pathway	Cell cycle

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* OriGene provides validated application data and protocol, with money back guarantee.

WB Image

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BUB1B (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BUB1B.

WB Image

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-BUB1B monoclonal antibody.

IHC Image

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-BUB1B mouse monoclonal antibody. (TA500541, Dilution 1:50)

IHC Image

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-BUB1Bmouse monoclonal antibody. (TA500541, Dilution 1:50)

IHC Image

Immunohistochemical staining of paraffin-embedded Carcinoma of liver tissue using anti-BUB1Bmouse monoclonal antibody. (TA500541, Dilution 1:50)

IHC Image

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-BUB1Bmouse monoclonal antibody. (TA500541, Dilution 1:50)

IF Image

Anti-BUB1B mouse monoclonal antibody (TA500541) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BUB1B(RC205844).