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Novel HIF2A mutations disrupt oxygen sensing, leading to polycythemia, paragangliomas, and somatostatinomas Blood, Mar 2013; 121: 2563 - 2566. [anti-HA]

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RNA elements directing in vivo assembly of the 7SK/MePCE/Larp7 transcriptional regulatory snRNP Nucleic Acids Res., Mar 2013; 10.1093/nar/gkt159. [LA]

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Home All anti-BUB1B antibodies

Anti-BUB1B TrueMAB Antibody clone 10B7

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Related Products Conjugation/Bulk FAQs
SKU Description Amount Price Availability*  
TA500535
  • Purified anti-BUB1B (BubR1) mouse monoclonal antibody, clone 10B7
  • FREE positive control: HEK293T cell transient overexpression lysate (LC420068) , 20ug
100ul $325 In Stock Add to Shopping Cart
CF500535
  • Carrier-free (BSA/glycerol-free) BUB1B mouse monoclonal antibody, clone 10B7
100ug $450 3-4 weeks Add to Shopping Cart
WB(1)
IHC(10)
IF(1)
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Also for BUB1B (NM_001211)
cDNA Clone shRNA/siRNA Lysate Protein Antibody
Gene NameHomo sapiens BUB1 mitotic checkpoint serine/threonine kinase B (BUB1B)
Synonyms:Bub1A; BUB1beta; BUBR1; hBUBR1; MAD3L; MVA1; SSK1
ImmunogenProtein expressed in 293T cell transfected with human BUB1B expression vector
BufferPBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Clone Nameclone 10B7 IsotypeIgG1
Species ReactivityHuman Concentration0.5~1.0 mg/ml (Lot Dependent)
Purification Purified from mouse ascites fluids by affinity chromatography (Protein A or G Sepharose)
Guaranteed Application *WB, IHC, IF Suggested DilutionsWB 1:2000 IHC 1:50 IF 1:100
BackgroundThis gene encodes a kinase involved in spindle checkpoint function. The protein has been localized to the kinetochore and plays a role in the inhibition of the anaphase-promoting complex/cyclosome(APC/C), delaying the onset of anaphase and ensuring proper chromosome segregation. Impaired spindle checkpoint function has been found in many forms of cancer.
Related Pathway
Cell cycle

* Shipping is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BUB1B (RC205844, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BUB1B.
IHC Image
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-BUB1B mouse monoclonal antibody. (TA500535, Dilution 1:50)
IHC Image
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-BUB1Bmouse monoclonal antibody. (TA500535, Dilution 1:50)
IHC Image
Immunohistochemical staining of paraffin-embedded Carcinoma of kidney tissue using anti-BUB1Bmouse monoclonal antibody. (TA500535, Dilution 1:50)
IHC Image
Immunohistochemical staining of paraffin-embedded Carcinoma of liver tissue using anti-BUB1Bmouse monoclonal antibody. (TA500535, Dilution 1:50)
IHC Image
Immunohistochemical staining of paraffin-embedded Carcinoma of lung tissue using anti-BUB1Bmouse monoclonal antibody. (TA500535, Dilution 1:50)
IHC Image
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-BUB1Bmouse monoclonal antibody. (TA500535, Dilution 1:50)
IHC Image
Immunohistochemical staining of paraffin-embedded Carcinoma of thyroid tissue using anti-BUB1Bmouse monoclonal antibody. (TA500535, Dilution 1:50)
IHC Image
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of endometrium tissue using anti-BUB1Bmouse monoclonal antibody. (TA500535, Dilution 1:50)
IHC Image
Immunohistochemical staining of paraffin-embedded Carcinoma of prostate tissue using anti-BUB1Bmouse monoclonal antibody. (TA500535, Dilution 1:50)
IHC Image
Immunohistochemical staining of paraffin-embedded Carcinoma of bladder tissue using anti-BUB1Bmouse monoclonal antibody. (TA500535, Dilution 1:50)
IF Image
Anti-BUB1B mouse monoclonal antibody (TA500535) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BUB1B(RC205844).

 

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