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Home Antibody All anti-NEK6 antibodies

Anti-NEK6 TRUEMAB Antibody clone 5B9

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA500529
  • NEK6 mouse monoclonal antibody, clone 5B9
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC402327) , 20ug
100ul 325 In Stock
CF500529
  • Carrier-free (BSA/glycerol-free) NEK6 mouse monoclonal antibody, clone 5B9
100ug $450 3-4 weeks
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WB(1)
IP(1)
IHC(6)
IF(1)
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Also for NEK6 (NM_014397)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human NEK6 (NP_055212) produced in HEK293T cell.
Clone Nameclone 5B9 IsotypeIgG1
Species ReactivityHuman Concentration1.50mg/ml
Guaranteed Application *WB, IHC, IF, IP Suggested DilutionsWB 1:1000, IHC 1:50, IF 1:100, IP 2ug/500ul
BufferPBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens NIMA-related kinase 6 (NEK6), transcript variant 2
Alternative NameSID6-1512
Database LinkNP_055212
FunctionThe Aspergillus nidulans 'never in mitosis A' (NIMA) gene encodes a serine/threonine kinase that controls initiation of mitosis. NIMA-related kinases (NEKs) are a group of protein kinases that are homologous to NIMA. Evidence suggests that NEKs perform functions similar to those of NIMA.[supplied by OMIM].
Related Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NEK6 (RC203609, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NEK6.
IHC Image
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-NEK6 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA500529)
IHC Image
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-NEK6 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA500529)
IHC Image
Immunohistochemical staining of paraffin-embedded Human Ovary tissue using anti-NEK6 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA500529)
IHC Image
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-NEK6 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA500529)
IHC Image
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-NEK6 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA500529)
IHC Image
Immunohistochemical staining of paraffin-embedded Human endometrium tissue using anti-NEK6 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA500529)
IF Image
Anti-NEK6 mouse monoclonal antibody (TA500529) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY NEK6(RC203609).
IP Image
Immunoprecipitation(IP) of NEK6 by using TrueMab monoclonal anti-NEK6 antibodies (Negative control: IP without adding anti-NEK6 antibody.). For each experiment, 500ul of DDK tagged NEK6 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-NEK6 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.

 

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