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Anti-SLC7A8 TRUEMAB Antibody Clone OTI5A9
TrueMAB Antibodies - Made against Authentic Protein Antigens
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Also for SLC7A8 (NM_012244)
|Full-length protein expressed in 293T cell transfected with human SLC7A8 expression vector .|
|WB, IHC, IF, IP, FC
||WB 1:500, IHC 1:50, IF 1:100, FLOW 1:100, IP 2ug/500ul
|PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
|Homo sapiens solute carrier family 7 (amino acid transporter light chain, L system), member 8 (SLC7A8), transcript variant 1|
Entrez Gene 23428 Human
|Sodium-independent, high-affinity transport of small and large neutral amino acids such as alanine, serine, threonine, cysteine, phenylalanine, tyrosine, leucine, arginine and tryptophan, when associated with SLC3A2/4F2hc. Acts as an amino acid exchanger. Has higher affinity for L-phenylalanine than LAT1 but lower affinity for glutamine and serine. L-alanine is transported at physiological concentrations. Plays a role in basolateral (re)absorption of neutral amino acids. Involved in the uptake of methylmercury (MeHg) when administered as the L-cysteine orD,L-homocysteine complexes, and hence plays a role in metal ion homeostasis and toxicity. Involved in the cellular activity of small molecular weight nitrosothiols, via the stereoselective transport of L-nitrosocysteine (L-CNSO) across the transmembrane. Plays an essential role in the reabsorption of neutral amino acids from the epithelial cells to the bloodstream in the kidney.|
|TransmembraneDruggable Genome |
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SLC7A8 (RC208586, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SLC7A8.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SLC7A8 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).(1:200)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-SLC7A8 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500503)
Anti-SLC7A8 mouse monoclonal antibody (TA500503) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SLC7A8(RC208586).
Immunoprecipitation(IP) of SLC7A8 by using TrueMab monoclonal anti-SLC7A8 antibodies (Negative control: IP without adding anti-SLC7A8 antibody.). For each experiment, 500ul of DDK tagged SLC7A8 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-SLC7A8 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
HEK293T cells transfected with either RC208586 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-SLC7A8 antibody(TA500503), and then analyzed by flow cytometry.