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Anti-MAPK1 TRUEMAB Antibody Clone OTI6F8
TrueMAB Antibodies - Made against Authentic Protein Antigens
Also for MAPK1 (NM_138957)
|Full length human recombinant protein of human MAPK1(NP_620407) produced in HEK293T cell.|
|Human, Dog, Rat, Monkey
|WB, IHC, IP, FC
||WB 1:1000~2000, IHC 1:50, FLOW 1:100, IP 2ug/500ul
|PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
|Homo sapiens mitogen-activated protein kinase 1 (MAPK1), transcript variant 2|
|ERK; ERK-2; ERK2; ERT1; MAPK2; p38; p40; p41; p41mapk; p42-MAPK; P42MAPK; PRKM1; PRKM2|
Entrez Gene 5594 Human
Entrez Gene 116590 Rat
Entrez Gene 698569 Monkey
Entrez Gene 477575 Dog
|The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. Two alternatively spliced transcript variants encoding the same protein, but differing in the UTRs, have been reported for this gene.|
|Protein KinaseDruggable Genome MAPK signaling pathwayErbB signaling pathwayChemokine signaling pathwayOocyte meiosismTOR signaling pathwayVascular smooth muscle contractionMore Pathways >> |
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAPK1 (RC204703, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAPK1.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-MAPK1 monoclonal antibody.
Western blot analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-MAPK1 monoclonal antibody.(1:200)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-MAPK1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500468)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-MAPK1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500468)
Immunoprecipitation(IP) of MAPK1 by using TrueMab monoclonal anti-MAPK1 antibodies (Negative control: IP without adding anti-MAPK1 antibody.). For each experiment, 500ul of DDK tagged MAPK1 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-MAPK1 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
HEK293T cells transfected with either RC204703 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-MAPK1 antibody(TA500468), and then analyzed by flow cytometry.