Anti-NRBP1 Antibody, clone 4C1

Also for NRBP1 (NM_013392)

OriGene Data

Immunogen	Protein expressed in 293T cell transfected with human NRBP1 expression vector
Clone Name	clone 4C1	Isotype	IgG2b
Species Reactivity	Human	Concentration	0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *	WB, IHC, IP, FC	Suggested Dilutions	WB 1:1000~2000, IHC 1:50, FLOW 1:100, IP 2ug/500ul
Buffer	PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification	Purified from mouse ascites fluids by affinity chromatography (Protein A or G Sepharose)

Reference Data

Target Name	Homo sapiens nuclear receptor binding protein 1 (NRBP1)
Alternative Name	BCON3; MADM; MUDPNP; NRBP
Database Link	NP_037524
Function	May play a role in subcellular trafficking between the endoplasmic reticulum and Golgi apparatus through interactions with the Rho-type GTPases. Binding to the NS3 protein of dengue virus type 2 appears to subvert this activity into the alteration of the intracellular membrane structure associated with flaviviral replication
Related Pathway

* Shipping is in business days

* OriGene provides validated application data and protocol, with money back guarantee.

WB Image

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NRBP1 (RC200107, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NRBP1.

WB Image

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-NRBP1 monoclonal antibody.

IHC Image

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-NRBP1 mouse monoclonal antibody. (TA500461)

IHC Image

Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-NRBP1 mouse monoclonal antibody. (TA500461)

IHC Image

Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-NRBP1 mouse monoclonal antibody. (TA500461)

IP Image

Immunoprecipitation(IP) of NRBP1 by using TrueMab monoclonal anti-NRBP1 antibodies (Negative control: IP without adding anti-NRBP1 antibody.). For each experiment, 500ul of DDK tagged NRBP1 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-NRBP1 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.

FC Image

HEK293T cells transfected with either RC200107 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-NRBP1 antibody(TA500461), and then analyzed by flow cytometry.

Conjugation Services
OriGene provides conjugation services of TrueMAB antibodies with the following molecules:

HRP
Biotin
PE
FITC
DyLight® 488
DyLight® 550
DyLight® 594
DyLight® 633

* DyLight is a trademark of Thermo-Fisher Scientific

Bulk Order
OriGene offers bulk sizes of TrueMAB antibodies in both the BSA/glycerol formulation and the carrier-free (BSA/glycerol-free) formulation.

Anti-NRBP1 TrueMAB Antibody clone 4C1

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

OriGene Data

Reference Data