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Home Antibody All anti-BRAF antibodies

Anti-BRAF TRUEMAB Antibody clone 3B12

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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SKU Description Amount Price Availability*  
TA500446
  • anti-BRAF mouse monoclonal antibody,clone 3B12
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC401382) , 20ug Explanation
100ul $325 In Stock
CF500446
  • Carrier-free (BSA/glycerol-free) BRAF mouse monoclonal antibody,clone 3B12
100ug $450 3-4 weeks
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WB(2)
IP(1)
IHC(4)

OriGene Data

ImmunogenFull-length protein expressed in 293T cell transfected with human BRAF expression vector
Clone Nameclone 3B12 IsotypeIgG1
Species ReactivityHuman, Dog, Monkey Concentration0.97 mg/ml
Guaranteed Application *WB, IHC, IP Suggested DilutionsWB 1:2000 IHC 1:50
Predicted MW Explanation 84.4 kDa
BufferPBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens B-Raf proto-oncogene, serine/threonine kinase (BRAF)
Alternative NameB-RAF1; BRAF1; NS7; RAFB1
Database LinkNP_004324
Entrez Gene 673 Human
Entrez Gene 475526 Dog
FunctionThis gene encodes a protein belonging to the raf/mil family of serine/threonine protein kinases.This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation,and secretion. Mutations in this ge
Related PathwayProtein KinaseDruggable Genome MAPK signaling pathwayErbB signaling pathwayChemokine signaling pathwaymTOR signaling pathwayVascular smooth muscle contractionFocal adhesionMore Pathways >>

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BRAF (RC211013, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BRAF.
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-anti-BRAFmonoclonal antibody.
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-BRAFmouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500446, Dilution 1:50)
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Immunohistochemical staining of paraffin-embedded Ovary tissue using anti-BRAFmouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500446, Dilution 1:50)
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Immunohistochemical staining of paraffin-embedded pancreas tissue using anti-BRAFmouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500446, Dilution 1:50)
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Immunohistochemical staining of paraffin-embedded endometrium tissue using anti-BRAFmouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500446, Dilution 1:50)
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Immunoprecipitation(IP) of BRAF by using TrueMab monoclonal anti-BRAF antibodies (Negative control: IP without adding anti-BRAF antibody.). For each experiment, 500ul of DDK tagged BRAF overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-BRAF antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
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