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Home All anti-BRAF antibodies
Anti-BRAF TrueMAB Antibody clone 3E8
TrueMAB™ Antibodies - Made against Authentic Protein Antigens
* Buy one primary antibody, get 50% off a GAPDH loading control antibody.
Also for BRAF (NM_004333)
OriGene Data
| Full length human recombinant protein of human BRAF (NP_004324) produced in HEK293T cell. |
| clone 3E8 |
IgG2b |
| Human |
0.5~1.0 mg/ml (Lot Dependent) |
| WB, IHC, IF |
WB 1:2000, IHC 1:50, IF 1:100, |
| PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
| Purified from mouse ascites fluids by affinity chromatography (Protein A or G Sepharose) |
Reference Data
| Homo sapiens v-raf murine sarcoma viral oncogene homolog B1 (BRAF) |
| B-RAF1; BRAF1; NS7; RAFB1 |
| NP_004324 |
| This gene encodes a protein belonging to the raf/mil family of serine/threonine protein kinases.This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation,and secretion. Mutations in this gene are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. Mutations in this gene have also been associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma,non-small cell lung carcinoma, and adenocarcinoma of lung. A pseudogene, which is located on chromosome X, has been identified for this gene. |
Focal Adhesion MAPK signaling pathway Senescence and Autophagy |
* Shipping is in business days
* OriGene provides validated application data and protocol, with money back guarantee.
WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BRAF (RC211013, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BRAF.
IHC Image
Immunohistochemical staining of paraffin-embedded Human colon tissue using anti-BRAF mouse monoclonal antibody. (TA500441)
IF Image
Anti-BRAF mouse monoclonal antibody (TA500441) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BRAF(RC211013).
Conjugation Services
OriGene provides conjugation services of TrueMAB antibodies with the following molecules:
- HRP
- Biotin
- PE
- FITC
- DyLight® 488
- DyLight® 550
- DyLight® 594
- DyLight® 633
* DyLight is a trademark of Thermo-Fisher Scientific
Bulk Order
OriGene offers bulk sizes of TrueMAB antibodies in both the BSA/glycerol formulation and the carrier-free (BSA/glycerol-free) formulation.
Contact us with your conjugation/bulk request: antibody@origene.com or 888-267-4436 (Tech Support)
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