Anti-TYRO3 Antibody, clone 9B12

Also for TYRO3 (NM_006293)

Gene Name	Homo sapiens TYRO3 protein tyrosine kinase (TYRO3)
Synonyms:	BYK; Dtk; RSE; Sky; Tif
Immunogen	Full length human recombinant protein of human TYRO3 (NP_006284) produced in HEK293T cell.
Buffer	PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Clone Name	clone 9B12	Isotype	IgG1
Species Reactivity	Human	Concentration	0.5~1.0 mg/ml (Lot Dependent)
Purification	Purified from mouse ascites fluids by affinity chromatography (Protein A or G Sepharose)
Guaranteed Application *	WB, IHC, IF, IP, FC	Suggested Dilutions	WB 1:2000, IHC 1:150, IF 1:100, FLOW 1:100, IP 2ug/500ul
Background	May be involved in cell adhesion processes, particularly in the central nervous system.In case of filovirus infection, seems to function as a cell entry factor.
Related Pathway

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WB Image

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TYRO3 (RC208260, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TYRO3.

IHC Image

Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-TYRO3 mouse monoclonal antibody. (TA500421)

IHC Image

Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-TYRO3 mouse monoclonal antibody. (TA500421)

IF Image

Anti-TYRO3 mouse monoclonal antibody (TA500421) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY TYRO3(RC208260).

IP Image

Immunoprecipitation(IP) of TYRO3 by using TrueMab monoclonal anti-TYRO3 antibodies (Negative control: IP without adding anti-TYRO3 antibody.). For each experiment, 500ul of DDK tagged TYRO3 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-TYRO3 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.

FC Image

HEK293T cells transfected with either RC208260 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-TYRO3 antibody(TA500421), and then analyzed by flow cytometry.

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Anti-TYRO3 TrueMAB Antibody clone 9B12

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