Anti-SSB Antibody, clone 3H9

Also for SSB (NM_003142)

OriGene Data

Immunogen	Protein expressed in 293T cell transfected with human SSB expression vector
Clone Name	clone 3H9	Isotype	IgG2a
Species Reactivity	Human , Dog	Concentration	0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *	WB, IHC, IF, IP, FC	Suggested Dilutions	WB 1:1000~2000, IHC 1:150, IF 1:100, FLOW 1:100, IP 2ug/500ul
Buffer	PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification	Purified from mouse ascites fluids by affinity chromatography (Protein A or G Sepharose)

Reference Data

Target Name	Homo sapiens Sjogren syndrome antigen B (autoantigen La) (SSB)
Alternative Name	La; LARP3
Database Link	NP_003133
Function	La is involved in diverse aspects of RNA metabolism, including binding and protecting 3-prime UUU(H) elements of newly RNA polymerase III (see MIM 606007)-transcribed RNA, processing 5-prime and 3-prime ends of pre-tRNA precursors, acting as an RNA chaperone, and binding viral RNAsassociated with hepatitis C virus. La protein was originally defined by its reactivity with autoantibodies from patients with Sjogren syndrome (MIM 270150) and systemic lupus erythematosus
Related Pathway

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WB Image

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SSB (RC205013, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SSB.

WB Image

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SSB monoclonal antibody.

IHC Image

Immunohistochemical staining of paraffin-embedded Human breast tissue using anti-SSB mouse monoclonal antibody. (TA500410)

IHC Image

Immunohistochemical staining of paraffin-embedded Human lung tissue using anti-SSB mouse monoclonal antibody. (TA500410)

IHC Image

Immunohistochemical staining of paraffin-embedded Human Ovary tissue using anti-SSB mouse monoclonal antibody. (TA500410)

IHC Image

Immunohistochemical staining of paraffin-embedded Human endometrium tissue using anti-SSB mouse monoclonal antibody. (TA500410)

IHC Image

Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-SSB mouse monoclonal antibody. (TA500410)

IF Image

Immunofluorescent staining of HT29 cells using anti-SSB mouse monoclonal antibody (TA500410).

IP Image

Immunoprecipitation(IP) of SSB by using TrueMab monoclonal anti-SSB antibodies (Negative control: IP without adding anti-SSB antibody.). For each experiment, 500ul of DDK tagged SSB overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-SSB antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.

FC Image

Flow cytometric Analysis of Jurkat cells, using anti-SSB antibody(TA500410),(Red), compared to a nonspecific negative control antibody,(Blue).

Conjugation Services
OriGene provides conjugation services of TrueMAB antibodies with the following molecules:

HRP
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PE
FITC
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DyLight® 594
DyLight® 633

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Bulk Order
OriGene offers bulk sizes of TrueMAB antibodies in both the BSA/glycerol formulation and the carrier-free (BSA/glycerol-free) formulation.

Anti-SSB TrueMAB Antibody clone 3H9

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

OriGene Data

Reference Data